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Cloning and Abiotic Expression Analysis of GmPHD3 Gene in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年04期
Page:
500-508
Research Field:
Publishing date:

Info

Title:
Cloning and Abiotic Expression Analysis of GmPHD3 Gene in Soybean
Author(s):
WANG Yi-long;  ZHAO Hai-hong;  SHEN Ying-zi;  QIAN Pei-pei;  MA Hao
(National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University， Nanjing 210095, China)
Keywords:
Soybean;  GmPHD3;  Expression analysis;  High temperature and humidity
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.04.0500
Abstract:
In order to investigate the regulatory role of soybean PHD-finger transcription factor family coding gene GmPHD3 in resisting high temperature and humidity abiotic stress in seed degradation in southern China, we isolated the full-length GmPHD3 gene and conduct bioinformatics analysis, subcellular localization and transcriptional activation analysis. We used leaves and cDNAs of pre-harvest seed deterioration resistance varieties Xiangdou 3 and deterioration varieties Ningzhen 1 as materials to perform the tissue expression pattern analysis and expression pattern analysis under high temperature and humidity stress with RT-PCR. The results of bioinformatics analysis showed that the length of CDS sequence was 738 bp, encoding 246 amino acids, containing two domains of Alfin and PHD-finger. The results of the phylogenetic tree indicated that the genetic distance of this gene was close to that of pigeon pea ALFIN-Like 3-like (XM_020363358.1). Subcellular localization results showed that the protein was expressed in the nucleus. Transcription activation test results showed that the gene had transcription activation activity in the full length, the activation domain was the N-terminal Alfin domain, and the C-terminal PHD-finger domain had no transcription activation activity. Analysis of tissue expression patterns revealed that the gene was mainly expressed at maturity and there was a difference between the two varieties. Analysis of the expression pattern under stress showed that the relative expression of the gene gradually increased with the extension of stress time. The research results lay a theoretical foundation for further elucidating the research on the regulation mechanism under high temperature and humidity stress.

References:

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Last Update: 2020-09-02