|Table of Contents|

Protective Effect of Soy Isoflavones on Injury of L02 Cells Induced by H2O2(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年03期
Page:
458-463
Research Field:
Publishing date:

Info

Title:
Protective Effect of Soy Isoflavones on Injury of L02 Cells Induced by H2O2
Author(s):
ZHENG Feng1; 2;  JIN Fang-duo1;  JIN Mei-hua1;  ZHANG Tian1;  CUI Xiang-dan2;  YIN Xue-zhe2;  QUAN Ji-shu1
(1.Medical College, Yanbian University Yanji 133002, China; 2.Yanbian University Hospital, Yanji 133000, China)
Keywords:
Soybean;  Isoflavones;  L02;  H2O2;  Protective effect
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.03.0458
Abstract:
In order to detect the protective effect of soy isoflavones (ISOF) on injury of L02 cells induced by hydrogen peroxide (H2O2), this study established the cellular injury model after the L02 cells were pretreatment with ISOF and induced with H2O2. The cell viability was investigated with cell counting kit-8 (CCK-8) assay, the medium activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and the cellular reduced glutathione (GSH) were tested with the microplate method, superoxide dismutase (SOD) activity was assayed with hydroxylamine method, the malondialdelyde (MDA) content was tested with the thibabituric acid (TBA) method. The results showed that H2O2 decreased the viability of L02 cells by 51%, and elevated the LDH leakage to culture medium, indicative of the establishment of oxidative injury model of L02 cells. ISOF had no cytotoxicity within the scope of the concentration of 10-40 mg?L-1. However, pretreatment with ISOF could alleviate the above-mentioned damage indicators of L02 cells. Compared with H2O2 injury group, the cell viability of L02 was increased by 28.9% (P<0.05), the leakage of LDH, ALT, AST was reduced by 91.7%, 91.7% and 74.1%, respectively, the cellular MDA was decreased by 118.5%, and the cellular GSH and SOD were increased by 184.4% and 76.2% respectively in the 40 mg?L-1 ISOF group. It is suggested that ISOF could protect L02 cells from oxidative damage induced by H2O2.

References:

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Last Update: 2020-07-14