|Table of Contents|

Systematic Identification of Fusarium Species Associated with Soybean Root Rot in Dunhua and Gongzhuling, Jilin Province(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年03期
Page:
442-450
Research Field:
Publishing date:

Info

Title:
Systematic Identification of Fusarium Species Associated with Soybean Root Rot in Dunhua and Gongzhuling, Jilin Province
Author(s):
SANG Yong-sheng1; 2;  LIU Yue1; 2;  YUAN Cui-ping2;  QIU Hong-mei2;  LIU Xiao-dong2;  WANG Yue-qiang2;  LI Yu-qiu2;  DONG Ying-shan1; 2
(1.College of Agriculture, Jilin Agricultural University, Changchun 130118, China; 2.Jilin Academy of Agricultural Sciences, Changchun 130033, China)
Keywords:
Soybean (Glycine max);  Root rot;  Fusarium;  Concatenated sequence;  Phylogeny
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.03.0442
Abstract:
In order to identify the fusarium species associated with soybean root rot in Dunhua and Gongzhuling,93 samples of diseased plants with typical root rot symptom were collected to isolate and identify the species of Fusarium causing the disease in Gongzhuling and Dunhua, Jilin province. Sequence alignment carried out by ITS, TEF1-α and RPB2 genes, multiple-gene phylogenetic analysis and morphology were used to identify the pathogens. 104 strains of Fusarium classified in 4 species were identified, including F. solani (64.42%), F. oxysporum (33.65%), F. verticillioides (0.96%) and F. proliferaum (0.96%). 57 out of the 104 strains of Fusarium were isolated from the diseased plants in Gongzhuling, including F. solani (91.23%), F. oxysporum (5.27%), F. verticillioides (1.75%) and F. proliferaum (1.75%). The other 47 strains were isolated from Dunhua, including F. solani (31.91%) and F. oxysporum (68.09%). The results showed F. solani and F. oxysporum were respectively the dominant pathogen in Gongzhuling and Dunhua. Pathogenicity test showed that only 3 strains of F. solani had no pathogenicity to Hefeng 55, but the other 101 strains had different degrees of pathogenicity to Hefeng 55.

References:

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Last Update: 2020-07-14