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Genetic Diversity Analysis of the Wild Soybean (Glycine soja) Populations in China by MSAP Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年03期
Page:
384-389
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of the Wild Soybean (Glycine soja) Populations in China by MSAP Method
Author(s):
YAN Xue-fei1;  GUO Wei1;  CAO Ying1;  WANG Yu-min2;  YUAN Cui-ping2;  ZHAO Hong-kun2;  DONG YING-shan2;  LIU Xiao-dong2; 3
（1.College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China; 2.Soybean Research Institute, Jilin Academy of Agricultural Science, Changchun 130033, China; 3.College of Agronomy, Jilin Agricultural University, Changchun 130118, China）
Keywords:
Wild soybean;  Genetic diversity;  Latitude;  MSAP;  Methylation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.03.0384
Abstract:
Seven wild soybean populations between latitude 27° and 46°N in China were the targets for analysis of genetic diversity. Methylation-sensitive amplification polymorphisms (MSAP) was applied to evaluate the DNA methylation patterns and genetic structure. The main results were as follows: A total of 1 617 loci were detected with a set of eight primer pairs in 223 materials. Pattern Ⅳ of DNA methylation had the highest percentage and occupied 56.6% of the total loci, while Pattern Ⅱ and Ⅲ had lower percentages (11.75%, 13.25%). Shannon index based on unmethylated loci had the lowest value in the population of Bayan (I=0.279 6), and with the increasing latitude, the genetic diversity rose gradually, which reached the highest value（I=0.313 0）in the population of Anhua. The population Bayan also had the lowest Shannon index value (I=0.585 1) based on methylation-susceptible loci, however, the population of Tongbai had the highest (I=0.603 0), which suggested no trend in epigenetic diversity with increasing latitude. The structure analysis showed that most of the materials were assigned to respective cluster based on unmethylated loci, which meant genetic differentiation existing in the populations. However, samples from population of Longjing, Wuqing, Yanggu and Tongbai tended to be clusted together based on methylation-susceptible loci. In the Mantel tests, correlations of the genetic distances with the epigenetic distances were high and statistically significant in the six populations, which suggested that epigenetic variation was to some degree dependent of variation in the DNA sequence.

References:

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Last Update: 2020-07-14