|Table of Contents|

Bioinformatics Analysis and Expression of Eight Dof Transcription Factors in Soybean Under Drought Stress(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年03期
Page:
377-383
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis and Expression of Eight Dof Transcription Factors in Soybean Under Drought Stress
Author(s):
LIU Bei1;  QIU Shuang2;  HE Jia-qi2;  LI Ming-yang2;  ZHAI Ying2
(1.College of Life Science, Changchun Sci-Tech University, Changchun 130600, China; 2.College of Life Science and Agro-Forestry, Qiqihar Univesity, Qiqihar 161006, China)
Keywords:
Soybean;  Seedling stage;  Dof transcription factor;  Drought stress;  Expression analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.03.0377
Abstract:
In order to explore the functions of soybean transcription factors Dof, the bioinformatics analysis was performed on eight Dof genes in soybean. The expression levels of them were detected through real-time fluorescence quantitative PCR under drought stress, and the cis-elements in promoter sequences of major responsive genes were also analyzed. The results showed that the eight genes encoding the Dof transcription factors located on chromosome 5, 8, 11, 13, 15 and 16 of soybean, respectively. The length of their proteins ranged from 213 to 403 amino acid residues, and the isoelectric points ranged from 6.61 to 9.36, mainly located in the nucleus. GmDof2 was closely related to tomato SlDof5.4, GmDof1, GmDof8, GmDof3 and GmDof5 were closely related to each other, GmDof7 was closely related to cucumber CsDof1.4, and GmDof4 was closely related to GmDof6. The expression levels of GmDof1 and GmDof3 increased obviously under drought stress, and their promoter sequences both contained several cis-elements related to stress. The results showed that soybean Dof transcription factors have potential application in genetic engineering of plant drought resistance.

References:

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Last Update: 2020-07-14