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Inheritance Analysis and Gene Mapping of Brown Seed Coat in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年03期
Page:
361-369
Research Field:
Publishing date:

Info

Title:
Inheritance Analysis and Gene Mapping of Brown Seed Coat in Soybean
Author(s):
DONG Quan-zhong1; 2;  JIANG Bing-jun3;  ZHANG Yong2;  XUE Hong2;  ZHANG Ming-ming2;  LI Wei-wei2;  HAN Tian-fu3;  NING Hai-long1
(1.Agronomy College, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education Key Laboratory of Soybean Biology and Breeding (Genetics), Ministry of Agriculture, Harbin 150030, China; 2.Keshan Branch of Academy of Heilongjiang Province, Keshan 161606, China; 3.Crop Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
Keywords:
Soybean;  Brown seed coat;  I gene;  Gene location
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.03.0361
Abstract:
In order to analyze the genetic rule and molecular basis of soybean seed coat mutation from yellow to brown, the mutant lines of brown seed coat (MLBSC) and its original parents of yellow seed coat (OPYSC) were evaluated in the field. Four pairs of MLBSC and OPYSC were genotyped by 176 simple sequence repeat (SSR) on 20 chromosomes. The positive cross and reverse crosses were mated between MLBSC and the yellow seed coat including OPYSC and the non-OPYSC, the statistical analysis on the segregation of seed number with two kinds of coat color in F1 and F2 was conducted. Genotyping of F2 individuals of cross Beidou 14×KeH 09-95 was conducted by 72 SSR markers in A2 linkage group. The results showed that the mutants of the brown seed coat were the isogenic lines of the original parents. Mutation of brown seed coat was heritable and was controlled by nuclear genes without domination of cytoplasmic inheritance. The yellow seed coat was dominant to the naturally mutated brown seed coat. By Chi-square test, the yellow and brown separation ratio of seed coat in the hybrid offspring was in accordance with the Mendelian independent genetic law of 3∶1 which was consistent with the genetic mode of classical genetics. The mutation occurred in the interval between sat_162 and SSR53 on A2 linkage group. The GmIRCHS structure (previously predicted to be I gene) has been found in the interval between SSR53 and sat_162 in the soybean common physical map (http://www.soybase.org). Summarizing above researches, the natural mutation of the classical I locus on A2 chromosome was the cause of the variation of seed coat color from yellow to brown. During the study, a new marker SSR53 related with seed color on the A2 chromosome was developed.

References:

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Last Update: 2020-07-14