|Table of Contents|

Establishment of qPCR and 3D-dPCR Detection Method of Genetically Modified Soybean MON87701(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年02期
Page:
212-219
Research Field:
Publishing date:

Info

Title:
Establishment of qPCR and 3D-dPCR Detection Method of Genetically Modified Soybean MON87701
Author(s):
WEN Hong-tao1; YANG Yang1; DING Yi-jia1; ZHANG Xiao-lei1; GUAN Hai-tao1; LI Liang2; ZHANG Rui-ying1
(1.Quality & Safety Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China； 2.Biotechnology Research Institute, Chinese Agricultural Academy of Sciences, Beijing 100081, China)
Keywords:
Genetically modified soybean; MON87701; Quantitative detection; qPCR; 3D-dPCR
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.02.0212
Abstract:
Genetically modified (GM) soybean event MON87701 is a commercial insect resistant soybean developed by Monsanto company, which has been widely used in 17 countries. In order to established efficient detection method of MON87701, this study established duplex quantitative detection method based on real-time fluorescence quantitive PCR (qPCR) and 3D digital PCR (3D-dPCR) platforms and tested 44 different transgenic materials. The result showed: The positive results were only obtained in the samples of MON87701, which confirmed that the primer and probe combination in the method had high specificity. The LOQs of the both methods were 0.09% and the LODs were 0.02%. There was no significant difference between the two methods. However, 3D-dPCR method does not need reference materials and the requirement on DNA quality is lower, it is more convenient and efficient providing new methods and ideas for the GMO detection.

References:

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Last Update: 2020-06-10