|Table of Contents|

Analysis on Characteristics of Mutant Population by 60Co-γ and EMSMutagenesis in Northeastern Soybean Cultivars(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年02期
Page:
174-182
Research Field:
Publishing date:

Info

Title:
Analysis on Characteristics of Mutant Population by 60Co-γ and EMSMutagenesis in Northeastern Soybean Cultivars
Author(s):
XUE Yong-guo1; LIU Xin-lei1; TANG Xiao-fei1; CAO Dan1; HU Shao-xin2; WANG Yong-bin3; WANG Guang-jin1; LUAN Xiao-yan1
(1.Soybean Research Institute, Heilongjiang Academy Agricultural of Science, Harbin 150086, China; 2.Institute of Corn, Heilongjiang Academy Agricultural of Science, Harbin 150086, China; 3.Institute of Biotechnology, Heilongjiang Academy Agricultural of Science, Harbin 150086, China)
Keywords:
Soybean;  60Co-γ radiation;  EMS;  Separated generations;  Mutation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.02.0174
Abstract:
Physical and chemical mutagenesis of soybean is an important way to obtain germplasm resources, and also an important means of soybean breeding. By comparing the mutagenic effects of different varieties, different mutagenic methods and doses, we can provide an effective way for soybean mutagenic breeding and mutant library construction. In this study, 60Co-γ radiation and EMS mutagenesis were carried out on four spring soybean varieties Heinong 48, Heinong 84, Suinong 52 and Suinong 42 in northeast China. The emergence rate, adult rate, sterility, protein, fat and other agronomic traits of M1 and M2 generation were investigated and analyzed. The results showed that the sensitivity of each variety of different mutagenesis methods was different. Heinong 48 was highly sensitive to radiation mutagenesis. Heinong 84, Suinong 52 and Suinong 42 were highly sensitive to chemical mutagenesis. Radiation mutagenesis and chemical mutagenesis of M1 generations can affect different traits of different varieties, but most of these effects were caused by physiological injury. The radiation mutagenesis had obvious post-seedling lethality, and the effect on sterility was more obvious. The chemical mutagenesis was mild, and the adult rate of M1 generation was higher. The emergence rate, adult rate, sterility, protein, fat and other agronomic traits of the two mutant M2 generations tend to be the same, but radiation mutagenesis were higher than chemical mutagenesis in leaf type, branching, yellowing plant and sterile line. The study of these characteristics can provide basis for mutagenesis breeding or mutant bank construction.

References:

［1］韩锁义,杨玛丽,陈远东,等.大豆“南农94-16”突变体库的构建及部分性状分析［J］.核农学报,2008,22(2):131-135.（Han S Y, Yang M L, Chen Y D, et al. Construction of mutant library for soybean Nannong 94-16 and analysis of some characters［J］. Journal of Nuclear Agricultural Sciences, 2008,22(2):131-135.)［2］Li Z, Jiang L, Ma Y, et al. Development and utilization of a new chemically-induced soybean library with a high mutation density［J］. Journal of Integrative Plant Biology, 2017, 59(1): 60-74.［3］Park S J, Buttery B R. Ethyl-methanesulphonate (EMS) induced nodulation mutants of common bean (Phaseolus vulgaris L.) lacking effective nodules［J］. Plant and Soil, 1992, 139(2): 295-298.［4］谢圣男, 王宏光, 杨振, 等. 大豆绥农14突变体库构建及株高性状分析［J］. 核农学报, 2018, 27(3):307-313. (Xie S N, Wang H G, Yang Z, et al. Construction of Suinong 14 mutant library and analysis of soybean height mutant［J］. Journal of Nuclear Agricultural Sciences, 2018, 27(3): 307-313.)［5］杨兆民, 张璐. 辐射诱变技术在农业育种中的应用与探析［J］. 基因组学与应用生物学, 2011, 30(1): 87-91. (Yang Z M, Zhang L. Radiation mutation breeding in agriculture technology application and analysis［J］. Genomics and Applied Biology, 2011, 30(1): 87-91.)［6］Bolon Y T, Stec A O, Michno J M, et al. Genome resilience and prevalence of segmental duplications following fast neutron irradiation of soybean［J］. Genetics, 2014, 198(3): 967-981.［7］徐明, 路铁刚. 植物诱变技术的研究进展［J］. 生物技术进展, 2011, 1(2): 90-97. (Xu M, Lu T G. Research progress of plant mutagenesis technology［J］. Current Biotechnology, 2011, 1(2): 90-97.)［8］张力伟, 樊颖伦, 牛腾飞, 等. 大豆“冀黄 13”突变体筛选及突变体库的建立［J］. 大豆科学, 2013, 32(1): 33-37. (Zhang L W, Fan Y L, Niu T F, et al. Screening of mutants and construction of mutant population for soybean cultivar “Jihuang 13” ［J］. Soybean Science, 2013, 32(1): 33-37.)［9］王连铮, 裴颜龙, 赵荣娟, 等, 大豆辐射育种的某些研究［J］. 中国油料作物学报, 2001, 23(2):1-5. (Wang L Z, Pei Y L, Zhao R J, et al. Some research on soybean mutation breeding［J］. Chinese Journal of Oil Crop Sciences, 2001, 23(2):1-5.)［10］陈远东, 喻德跃. EMS诱发大豆“南农94-16”突变体库的扩建及部分突变体的SSR分析［J］. 大豆科学, 2009, 22(12): 574-582. (Chen Y D, Yu Y D. Construction of mutant pools for soybean “Nannong 94-16” induced by EMS and analysis of SSR marker on several mutants［J］.Soybean Science, 2009, 22(12): 574-582.)［11］Bolon Y T, Haun W J, Xu W W, et al. Phenotypic and genomic analyses of a fast neutron mutant population resource in soybean［J］. Plant Physiology, 2011, 156(1): 240-253.［12］张小明, 薛永国.大豆突变体库构建研究进展［J］.大豆科学,2016,35(2): 345-350. (Zhang X M, Xue Y G. Research progress of soybean mutant library construction［J］. Soybean Science, 2016,35(2): 345-350.［13］翁秀英, 王彬如, 吴承礼, 等. 大豆辐射育种的研究［J］. 遗传学报, 1974, 1(2): l57-169. (Weng X Y, Wang B R, Wu C L, et al. Research on soybean mutation breeding［J］. Acta Genetica Sinica, 1974, 1(2): 157-169.)［14］薛永国, 魏崃, 唐晓飞, 等. 黑龙江省育成大豆品种性状演变的分析［J］. 大豆科学, 2015, 34(3): 361-366.（Xue Y G, Wei L,Tang X F, et al. Analysis and evolution on different traits of soybean varieties from Heilongjiang province［J］. Soybean Science 2015, 34(3): 361-366.)［15］降云峰, 刘永忠, 李万星, 等. 甲基磺酸乙酯诱变技术在大豆育种上的应用［J］. 园艺与种苗, 2012(6): 12-15.（Xiang Y F, Liu Y Z, Li W X, et al. Application of EMS inducing mutation technique on soybean breeding［J］. Harticulture and Seed,2012(6):12-15.)［16］孙玉, 姜永平, 刘军民. 我国大豆辐射诱变育种研究进展与展望［J］. 山东农业科学, 2008, 40(1): 14-17. (Sun Y, Jiang Y P, Liu J M. Achievement and prospect of soybean irradiation breeding in China［J］. Shandong Agricultural Sciences, 2008, 40(1): 14-17. )

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Last Update: 2020-06-10