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Study of Scanning Electron Microscopic Observation on the Mycelium Infection Process of Soybean Sclerotinia sclerotirum on Leaf and Stem(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年01期
Page:
125-129
Research Field:
Publishing date:

Info

Title:
Study of Scanning Electron Microscopic Observation on the Mycelium Infection Process of Soybean Sclerotinia sclerotirum on Leaf and Stem
Author(s):
SUN Ming-ming1; 2; LYU Shi-xiang2; LI Zhi-yuan2; WANG Ping3; WANG Guan2; ZHAO Xue1; HAN Ying-peng1; LI Wen-bin1
(1.Agronomy College/Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Breeding(Genetics) of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin 150030, China; 2.Heilongjiang Journal Press of Agricultural Science and Technology, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 3.Institute of High Education,Huaiyin Institute of Technology, Huai′an 223003，China)
Keywords:
Soybean;  Sclerotinia sclerotirum;  Mycelium;  Infection;  SEM
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.01.0125
Abstract:
In order to fully understand the mycelium infection process of soybean Sclerotinia sclerotirum and promote the selection of resistant varieties and the pathogenicity mechanism study, this study used the susceptible variety Hefeng 25 and the partial resistant variety Mapple Arrow as materials to systematically observe the characteristics of mycelium, the changes of stem and leaf tissue, and the infection characteristics after inoculating mycelium on the leaves and stem in vitro. The results showed that there were significant differences in the infection speed of mycelium and the size of tissue lesion among different resistant varieties. After inoculation, the mycelium produced branching and propagation rapidly, the tip shape of the branch grew elliptic and expanded, similar to the structure of ‘infection cell’. Five days after inoculation, the mycelium lacked nutrition source, the growth vigor decreased, the tissue dried up, and the infection ability decreased. On the third day after inoculation of mycelium, Hefeng 25, a susceptible variety, the surface materials of leaves almost disappeared, and the mesophyll cells shruncked obviously. The surface materials of the leaves of Mapple Arrow, a partial resistant variety, were basically intact, except for some cells which were sunken and shrunk, the shape of the rest cells were still relatively intact. On the third day, the observation performance of culms was similar to that of leaves, the surface materials of susceptible varieties disappeared, and that of resistant varieties was still basically reserved. In the process of leaf and stem infection, stomata invade the passage directly, and the mycelium could also invade the inside of tissue cells.

References:

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Last Update: 2020-03-17