|Table of Contents|

Screening of Drought-Tolerant Soybean Rhizobium and Its Symbiotic Compatibility Verification(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年01期
Page:
90-96
Research Field:
Publishing date:

Info

Title:
Screening of Drought-Tolerant Soybean Rhizobium and Its Symbiotic Compatibility Verification
Author(s):
WANG Peng-hui1; 2; JIANG Xin1; 2; MA Ming-chao1; 2; GUAN Da-wei1; 2; CAO Feng-ming1; 2; LIU Yao3;  KANG Yao-wei4;  LI Jun1; 2
(1.Institute of Agricultural Resources and Agricultural Regional Planning，Chinese Academy of Agricultural Sciences，Beijing 10081，China; 2.Laboratory of Quality & Safety Risk Assessment for Microbial Products (Beijing), Ministry of Agriculture, Beijing 100081, China; 3.National Center of Science and Technology Evaluation，MOST，Beijing 100081，China; 4.College of Life Science, Zhaoqing University, Zhaoqing 526061, China.)
Keywords:
Drought-tolerant;  Soybean rhizobium;  Major soybean cultivar;  Compatibility
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.01.0090
Abstract:
In order to solve the problem that the dry environment of soybean seeds that coated on the surface might lead to the rapid death of rhizobium, which limited the application prospect of rhizobium. In this study, 100 strains of soybean rhizobia isolated from different soybean producing areas in China were used as test materials, and the commercial strains B.diazoefficiens USDA110 and B.japonicom USDA6 were used as reference strains.The superior drought-resistance and nitrogen fixation strain were screened by the glass bead-drying method and vermiculite potting experiments. After drying for 24 h, the survival rate of strain 5873 was 0.44%, which was significantly higher than other soybean rhizobium and the control strains USDA110 and USDA6, indicating that the strain had better drought-tolerant characteristic. Analysis based on the 16S rDNA sequence phylogenetic analysis and ANI calculation showed the strain 5873 belonged to the Bradyrhizobium japonicom. The vermiculite potting experiments showed that the strain 5873 could nodulate with the nine main soybean varieties, which proved that the strains 5873 have extensive-host advantage in symbiotic nodulation.However,compared with the non-inoculation control, the nitrogen fixation ability were significantly different among different varieties. The dry weight, total nitrogen and chlorophyll of the plants that treated with rhizobium increased, expecially Diandou 5, Xudou 18 and Jidou 17.In this study, a soybean rhizobium strain with good resistance to drying and biological nitrogen fixation was screened and obtained. It can be used as a coating material for subsequent production, this study also provides a guarantee for the application of soybean rhizobium coating technology in Huanghuaihai and southern China.

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Last Update: 2020-03-17