|Table of Contents|

Identification of Soybean Resistance to Phytophthora sojae in the Germplasm Resources from Huanghuaihai Region of China(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年01期
Page:
12-22
Research Field:
Publishing date:

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Title:
Identification of Soybean Resistance to Phytophthora sojae in the Germplasm Resources from Huanghuaihai Region of China
Author(s):
YANG Jin WANG Xiao-men YE Wen-wu ZHENG Xiao-bo WANG Yuan-chao
(College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China)
Keywords:
Root rot Phytophthora sojae Soybean germplasm resources Disease resistance identification Resistance genes Huanghuaihai region
PACS:
-
DOI:
DOI:10.11861/j.issn.1000-9841.2020.01.0012
Abstract:
To identify the resistance level of the soybean cultivars(lines) from Huanghuaihai region to Phytophthora sojae (P. sojae) systematically, we used 381 soybean cultivars (lines) collected in 2017 and 2018 from various soybean breeding institutes, 8 representative P. sojae isolates, and a modified etiolated hypocotyl inoculation method. We found that 22 cultivars (lines) showed resistance to the strong virulent strain PsJS2, and more than 90% cultivars (lines) were resistant to the weak virulent strains Ps1, Ps3 and Ps5. All the cultivars (lines) exhibited 36 resistant/susceptible reaction patterns. The reaction patterns of target cultivars (lines) were compared with the differential lines to the 8 isolates. The reaction pattern occurring in 63 cultivars (lines) was consistent with that in the differential line carrying Rps1b, 36 cultivars (lines) produced reaction pattern coincide with Rps3a and 15 were postulated that contained Rps1d. The other 32 reaction patterns produced by 255 cultivars (lines), not consistent with any known differential line, may contain new Rps gene(s). Therefore, we found abundant resistant cultivars (lines) and diversity in Henan, Hebei, Beijing, Anhui, Shandong and Shanxi used a quickly method in greenhouse. Henan and Shandong possess abundant soybean cultivars (lines) containing Rps1b, Hebei and Beijing possess abundant soybean cultivars (lines) containing Rps3a. The data provided useful information for germplasm sources deployment during soybean breeding.

References:

[1]Sugimoto T, Kato M, Yoshida S, et al. Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans[J]. Breeding Science, 2012, 61(5):511-522.[2] Tyler B M. Phytophthora sojae: Root rot pathogen of soybean and model oomycete[J]. Molecular Plant Pathology, 2007, 8(1):1-8.[3] Doupnik B. Soybean production and disease estimates 1989 to 1991[J]. Plant Disease, 1993, 77(11):1170-1172.[4] 蔡莹莹, 宫千淳, 屈晓泽. 大豆疫霉根腐病的综合防治措施[J]. 生物化工, 2017, 3(3):62-63. (Cai Y Y, Gong Q C, Qu X Z. Comprehensive control measures of Phytophthora root rot of soybean[J]. Biological Chemical Engineering, 2017, 3(3):62-63.)[5] Schmitthenner A F. Problems and progress in control of Phytophthora root rot of soybean[J]. Plant Disease, 1985, 69(4):362-368.[6] Dorrance A E, Jia H, Abney T S. Evaluation of soybean differentials for their interaction with Phytophthora sojae[J/OL]. Plant Health Progress[2004-03-09]. Doi:10.1094/PHP2004-0309-1001-RS.[7] Sandhu D, Schallock K G, Rivera-Velez N, et al. Soybean Phytophthora resistance gene Rps8 maps closely to the Rps3 region[J]. Journal of Heredity, 2005, 96(5):536-541.[8] Wu X L, Zhang B Q, Sun S, et al. Identification, genetic analysis and mapping of resistance to Phytophthora sojae of Pm28 in soybean[J]. Agricultural Sciences in China, 2011, 10(10):1506-1511.[9] Zhang J Q, Xia C J, Duan C X, et al. Identification and candidate gene analysis of a novel Phytophthora resistance gene Rps10 in a Chinese soybean cultivar[J]. PLoS One, 2013, 8(7):e69799.[10]Ping J Q, Fitzgerald J C, Zhang C B, et al. Identification and molecular mapping of Rps11, a novel gene conferring resistance to Phytophthora soja in soybean[J]. Theoretical and Applied Genetics, 2016, 129(2):445-451.[11]Sahoo D K, Abeysekara N S, Cianzio S R, et al. A novel Phytophthora sojae resistance Rps12 gene mapped to a genomic region that contains several Rps genes[J]. PLoS One, 2017, 12(1):e0169950.[12]范爱颖, 王晓鸣, 方小平, 等. 大豆品种豫豆25抗疫霉根腐病基因的鉴定[J]. 作物学报, 2009, 35(10):1844-1850. (Fan A Y, Wang X M, Fang X P. et al. Molecular identification of Phytophthora resistance gene in soybean cultivar Yudou 25[J]. Acta Agronomica Sinica, 2009, 35(10):1844-1850.)[13]Sun S, Wu X L, Zhao J M, et al. Characterization and mapping of RpsYu25, a novel resistance gene to Phytophthora sojae[J]. Plant Breeding, 2011, 130(2):139-143.[14]Zhang J Q, Sun S L, Wang G Q, et al. Characterization of Phytophthora resistance in soybean cultivars/lines bred in Henan province[J]. Euphytica, 2014, 196(3): 375-384.[15]Li L, Lin F, Wang W, et al. Fine mapping and candidate gene analysis of two loci conferring resistance to Phytophthora sojae in soybean[J]. Theoretical and Applied Genetics, 2016, 129(12): 2379-2386.[16]Sun J T, Li L H, Zhao J M, et al. Genetic analysis and fine mapping of RpsJS, a novel resistance gene to Phytophthora sojae in soybean [Glycine max (L.) Merr][J]. Theoretical and Applied Genetics, 2014, 127(4):913-919.[17]于安亮, 徐鹏飞, 王金生, 等. 大豆品种绥农10抗疫霉根腐病遗传分析及抗病基因的SSR标记[J]. 中国油料作物学报, 2010, 32(4):462-466. (Yu A L, Xu P F, Wang J S, et al. Genetic analysis and SSR mapping of gene resistance[J]. Chinese Journal of Oil Crop Sciences, 2010, 32(4):462-466.)[18]Zhu Z D, Huo Y L, Wang X M, et al. Molecular identification of a novel Phytophthora resistance gene in soybean[J]. Acta Agronomica Sinica, 2007, 33(1):154-157.[19]Zhong C, Sun S, Yao L, et al. Fine mapping and identification of a novel Phytophthora root rot resistance locus RpsZS18 on chromosome 2 in soybean[J]. Frontiers in Plant Science, 2018, 9(44): 1-14.[20]Gao H Y, Narayanan N N, Ellison L, et al. Two classes of highly similar coiled coil-nucleotide binding-leucine rich repeat genes isolated from the Rps1-k locus encode Phytophthora resistance in soybean[J]. Molecular Plant-Microbe Interactions, 2005, 18(10):1035-1045.[21]Lohnes D G, Nickell C D, Schmitthenner A F. Origin of soybean alleles for Phytophthora resistance in China[J]. Crop Science, 1996, 36:1689-1692.[22]朱振东, 霍云龙, 王晓鸣, 等. 大豆疫霉根腐病抗源筛选[J]. 植物遗传资源学报, 2006, 7(1):24-30. (Zhu Z D, Huo Y L, Wang X M, et al. Screening for resistance sources to Phytophthora root rot in soybean [J]. Journal of Plant Genetic Resources. 2006, 7(1):24-30.)[23]李晓那, 孙石, 钟超, 等. 黄淮海地区大豆主栽品种对8个大豆疫霉菌株的抗性评价[J]. 作物学报, 2017, 43(12):1774-1783. (Li X N, Sun S, Zhong C, et al. Resistance evaluation to eight Phytophthora sojae isolates for major soybean cultivars in Huang-Huai-Hai rivers valley[J]. Acta Agronomica Sinica, 2017, 43(12): 1774-1783.)[24]Dorrance A E, Kurle J, Robertson A E, et al. Pathotype diversity of Phytophthora sojae in eleven states in the United States[J]. Plant Disease, 2016, 100(7):1429-1437.[25]Dong S, Qutob D, Tedman-Jones J, et al. The Phytophthora sojae avirulence locus Avr3c encodes a multi-copy RXLR effector with sequence polymorphisms among pathogen strains[J]. PLoS One, 2009, 4(5):e5556.[26]Kyle D E, Nickell C D, Nelson R L, et al. Response of soybean accessions from provinces in southern China to Phytophthora sojae[J]. Plant Disease, 1998, 82:555-559.[27]陈晓玲, 朱振东, 王晓鸣, 等. 大豆品种(系)抗疫霉根腐病基因推导[J]. 中国农业科学, 2008, 41(4):1227-1234.(Chen X L, Zhu Z D, Wang X M, et al. Postulation of Phytophthora resistance genes in soybean cultivars or lines[J]. Scientia Agricultura Sinica, 2008, 41(4):1227-1234.)[28]Huang J, Guo N, Li Y H, et al. Phenotypic evaluation and genetic dissection of resistance to Phytophthora sojae in the Chinese soybean mini core collection[J]. BMC Genet, 2016, 17:85.[29]Tang Q, Cui L, Li D, et al. Resistance evaluation of soybean germplasm from Huanghuai region to Phytophthora root rot[J]. Agricultural Sciences in China, 2011, 10(2):246-251.[30]崔林开. 中国大豆疫霉菌毒性组成、遗传结构及其无毒基因多态性的研究[D]. 南京:南京农业大学, 2010:36-43. (Cui L K. Study on virulence composition, genetic structure and polymorphism of Avr genes in Phytophthora sojae in China[D]. Nanjing: Nanjing Agricultural University, 2010: 36-43.[31]唐庆华, 崔林开, 李德龙, 等. 黄淮地区大豆种质资源对疫霉根腐病的抗病性评价[J]. 中国农业科学, 2010, 43(11):2246-2252. (Tang Q H, Cui L K, Li D L, et al. Resistance evaluation of soybean germplasm from Huanghuai Valley to Phytophthora root rot[J]. Scientia Agricultura Sinica, 2010, 43(11):2246-2252.)

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Last Update: 2020-03-17