|Table of Contents|

Effects of Daidzein and Its Phenylsulfonate Derivatives on Proliferation and Apoptosis of Lung Cancer Cells(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年06期
Page:
934-942
Research Field:
Publishing date:

Info

Title:
Effects of Daidzein and Its Phenylsulfonate Derivatives on Proliferation and Apoptosis of Lung Cancer Cells
Author(s):
WANG Xiao-bo1;  XIONG Xiao-wei1;  ZHU Yu-hua1; 3; PENG You2; WANG Xin1; HUANG Shao-xin1
(1.School of Basic Medical Science, Jiujiang University, Jiujiang 332000, China; 2.Jiangxi Province Engineering Research Center of Ecological Chemical Industry, Jiujiang University, Jiujiang 332005,China； 3.Clinical College of Medicine, Jiujiang University, Jiujiang 332000,China)
Keywords:
Daizein;  Phenylsulfonate derivatives;  Lung cancer cells;  Proliferation;  Apoptosis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.06.0934
Abstract:
In order to study the effects of daidzeint and their three phenysulfonate derivatives on proliferation and apoptosis of non-small cell lung cancer （NSCLC）cells, in vitro experiments were conducted on the treatment of A549, H1299 and HELF cells by daidzein and their three derivatives with 1.0×10-5-1.0×103 μmol·L-1 and 0-24 h treatment. CCK-8 was used to detect cell activity and apoptosis was detected by Tunel assay. RT-qPCR was used to verify the speculate that these effects maybe involved in TP53 and Caspase9. The results showed that, for the concentration range of 1×10-5-1×103 μmol·L-1, when the concentration of daidzein and derivative B was increased 10 times repectively, the proliferation of lung cancer cells could be inhibited to 3.26% (P<0.001), 5.23% (P<0.001) in A549 cells and 3.33% (P<0.001), 4.32%（P<0.001）in H1299 cells, while they were promoted 16.11% ［95% CI 13.74,18.49］(P<0.001), 9.92% (P<0.001) in HELF cells. By treatment with 10 μmol·L-1 of daidzein and derivative B from 0 to 24 h, HELF cells were promoted to 1.87%（P<0.001）, 1.72%（P<0.001）for every hour extent, while A549 cells were inhibited to 1.30%（P<0.001）, 1.46%（P<0.001） and H1299 cells were inhibited to 1.75%（P<0.001）, 2.07%（P=0.001）respectively. The relative counts of HELF cells increased by 42.66%（P=0.03）, and the counts of H1299 cells decreased by 18.33%（P=0.043）and A549 cells decreased by 20.67%（P=0.035）, when treated with 10 μmol·L-1 derivative B for 6 h. 10 μmol·L-1 derivative B could promote A549 and H1299 cells to apoptosis with 1.15 times（P<0.001） and 1.24 times (P=0.001), and protect HELF cells out of apoptosis with 0.84 times（P=0.005）. The mRNA expression of TP53and Caspase9were up-regulation in three cells. To a conclusion, daidzein and their 3 derivatives can inhibit the proliferation of lung cancer cells, and the effect is related to the concentration and time of treatment. derivative B can promote the apoptosis of lung cancer cells. The proliferation of lung cancer cells may be associated with the induction of apoptosis such as TP53and Caspase9.

References:

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Last Update: 1900-01-01