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¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2019Äê06ÆÚ

Page:

906-913

Research Field:

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Title:

Screening AGL15Mutant in Soybean by TILLING

Author(s):

SUN Yan-bo1; 2;  LI Zhong-feng3;  WANG Jun1;  QIU Li-juan1; 2

(1.College of Agriculture, Yangtze University, Jingzhou 434025, China; 2.Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Science/National Key Facility for Gene Resources and Genetic Improvement, Beijing 100081, China; 3.College of Agriculture, Henan Agricultural University, Zhengzhou 450002, China)

Keywords:

Soybean;  Transcription factor;  AGL15;  EMS;  TILLING

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2019.06.0906

Abstract:

AGL15is a member of the MADS (MCM1-AGAMOUS-DEFICIENS-SRF) gene family. MADS proteins regulate the accumulation of storage substances in plant seeds by binding specifically to cis-acting elements of downstream gene promoters. To verify the function of AGL15gene in soybean, eight GmAGL15mutants were screened from Mª­5 of ZP661 mutant induced by EMS with TILLING. Ten mutation sites were detected, six of which occurred in the coding region of the gene, resulting in changes in amino acids, and four occurred in the intron of the gene. One SNP changed from C to T at the 54th base of the second exon, and mutated the 79th amino acid from alanine to valine. Five SNPs occurred on the 112 base of the sixth exon, which changed the base from T to A, resulting in the mutation of the 568th amino acid from leucine to methionine. The mutation frequency of soybean GmAGL15was 1/805 kb. The protein or fat contents of five non-synonymous mutants and one intron mutant were significantly different from those of wild type ZP661. Allelic variation found in this study is helpful to elucidate the mechanism of GmAGL15in soybean seeds and provide valuable germplasm resources for soybean genetic improvement.

References:

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