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Cloning and Expression Analysis of GmAMS Gene and Its Promoter in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年06期
Page:
889-897
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of GmAMS Gene and Its Promoter in Soybean
Author(s):
JIN Ling;  ZHANG Hao;  WANG Song-ming;  LI Qiang;  DING Xian-long; YANG Shou-ping
（Soybean Research Institute, Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory for Biology and Genetic Improvement of Soybean(General), Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Modern Crop Production Collaborative Innovation Center, Nanjing 210095, China）
Keywords:
Soybean;  GmAMS gene; Promoter;  Gene cloning;  Expression analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.06.0889
Abstract:
The basic Helix-Loop-Helix(bHLH) transcription factor plays an important role in the regulation of male gamete development in plants. In order to explore the role of bHLH transcription factor in the flower development of soybean, the transcription factor GmAMS gene with typical bHLH domain and its promoter were cloned by RT-PCR using cDNA from the flower bud and DNA from the leaf of soybean cytoplasmic male sterile line NJCMS5A as the template. Bioinformatics analysis and spatiotemporal expression analysis of GmAMS gene were conducted. The results of bioinformatics analysis showed that the coding region sequence (CDS) of GmAMS gene was 1 716 bp in length, encoding 571 amino acids. Subcellular localization prediction results showed that GmAMS was localized in the nucleus. Real-time fluorescence quantification analysis showed that the expression level of GmAMS gene in the flower bud of NJCMS5A was significantly downregulated compared with the maintainer line NJCMS5B. Histochemical staining results showed that the GUS protein driven by GmAMS promoterwas mainly expressed at the early anther development stage.The above results provided the foundation for further study on the biological function and regulation mechanism of GmAMS gene in the flower development of soybean.

References:

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Last Update: 1900-01-01