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Transcriptome Analysis of Soybean Leaves Under the Stress of Pseudomonas syringae(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年06期
Page:
879-888
Research Field:
Publishing date:

Info

Title:
Transcriptome Analysis of Soybean Leaves Under the Stress of Pseudomonas syringae
Author(s):
ZHANG Xiong; XU Lu-rong; LIU Si-cen; ZHANG Zhen-chuan; LIU Zhi-cheng; GENG Xue-qing
（School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240,China）
Keywords:
Soybean; Pseudomonas syringae; Pto（AvrB）; RNA-seq; Differential expressed genes
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.06.0879
Abstract:
AvrB is one of the type III effector proteins secreted by Pseudomonas syringae pv. Glycinea, in order to study the changes of gene expression profile of soybean under biotic stress, we analyzed the difference of gene expression profiles of soybean leaves treated by different strains of Pto and Pto(AvrB)with RNA-seq technologyinsoybean Williams 82. And we explored whether the heterologous effector protein AvrB carried by the non-host pathogen Pseudomonas syringae pv. tomato can increase the pathogenicity of non-host pathogens.A total of 43 422 unigenes were screened out,37 611 common sequences between different treatments were coincident, and the AvrB treat group has the most sequence information. According to GO function analysis, genes can be divided into three major categories: Molecular function, cellular composition and biological process. The differentially expressed genes(DEGs) are widely involved in processes such as information transmission, immunity, and secondary metabolism. The KEGG pathway enrichment analysis indicated that the differentially expressed genes were mainly enriched in pathways such as plant hormone signal transduction, isoflavone biosynthesis, phenylalanine metabolism, and plant-pathogen interaction. The expression levels of four randomly selected DEGs in the RT-PCR were in conformity with the RNA-Seq data, confirming the reliability of the RNA-Seq results.The research promotes us to understand the pathogenic mechanism of type III effector protein factor promoting pathogenic bacteria，andprovides a basisforcultivating disease-resistant soybean varieties.

References:

［1］Zou J, Rodriguez-Zas S, Aldea M, et al. Expression profiling soybean response to Pseudomonas syringae reveals new defense-related genes and rapid HR-specific downregulation of photosynthesis［J］. Molecular Plant-Microbe Interactions, 2005, 18(11): 1161-1174.［2］Collmer A, Charkowski A O, Deng W L, et al. Bacterial Avr proteins: Secreted agents of parasitism and elicitors of plant defense［J］. Delivery and Perception of Pathogen Signals in Plants, 2001: 36-45.［3］White F F, Yang B, Johnson L B. Prospects for understanding avirulence gene function［J］. Current Opinion in Plant Biology, 2000, 3(4): 291-298.［4］Ashfield T, Ong L E, Nobuta K, et al. Convergent evolution of disease resistance gene specificity in two flowering plant families［J］. Plant Cell, 2004, 16(2): 309-318.［5］Grant M R, Godiard L, Straube E, et al. Structure of the Arabidopsis RPM1gene enabling dual specificity disease resistance［J］.Science, 1995, 269(5225): 843-846.［6］Mackey D, Iii B F H, Wiig A, et al. RIN4 interacts with Pseudomonas syringae type III effector molecules and is required for RPM1-mediated resistance in Arabidopsis［J］.Cell, 2002, 108(6): 743-754.［7］Shang Y L, Li X Y, Cui H T, et al. RAR1, a central player in plant immunity, is targeted by Pseudomonas syringae effector AvrB［J］. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(50): 19200-19205.［8］Cui H, Wang Y, Xue L, et al. Pseudomonas syringae effector protein AvrB perturbs Arabidopsis hormone signaling by activating MAP kinase 4［J］. Cell Host & Microbe, 2010, 2010, 7(2): 164-175.［9］Eitas T K, Nimchuk Z L, Dangl J L. Arabidopsis TAO1 is a TIR-NB-LRR protein that contributes to disease resistance induced by the Pseudomonas syringae effector AvrB［J］. Proceedings of the National Academy of Sciences, 2008, 105(17):6475-6480.［10］Selote D, Robin G P, Kachroo A. GmRIN4 protein family members function nonredundantly in soybean race-specific resistance against Pseudomonas syringae［J］. New Phytologist, 2013, 197(4):1225-1235.［11］Selote D, Kachroo A. RPG1-B-derived resistance to AvrB-expressing Pseudomonas syringae requires RIN4-like proteins in soybean［J］. Plant Physiology, 2010, 153(11):1199-211.［12］Leister R T, Katagiri F. A resistance gene product of the nucleotide binding site-Leucine rich repeats class can form a complex with bacterial avirulence proteins in vivo［J］. The Plant Journal, 2000, 22(4):345-354.［13］Selote D, Kachroo A. RIN4-like proteins mediate resistance protein-derived soybean defense against Pseudomonas syringae［J］. Plant Signaling & Behavior,2010,5(11):1453-1456.［14］Belkhadir Y. Arabidopsis RIN4 negatively regulates disease resistance mediated by RPS2 and RPM1 downstream or independent of the NDR1 signal modulator and is not required for the virulence functions of bacterial type III effectors AvrRpt2 or AvrRpm1［J］. The Plant Cell Online, 2004, 16(10):2822-2835.［15］Berretta J, Morillon A. Pervasive transcription constitutes a new level of eukaryotic genome regulation［J］. Embo Reports, 2009, 10(9): 973-982.［16］成舒飞,端木慧子,陈超,等.大豆MYB转录因子的全基因组鉴定及生物信息学分析［J］. 大豆科学,2016, 35(1): 52-57. (Cheng S F, Duanmu H Z,Cheng C,et al.Whole genome identification of soybean MYB transcription factors and bioinformatics analysis［J］. Soybean Science,2016, 35(1): 52-57.）［17］张峰, 赵云霞, 黄先忠. 利用转录组测序分析大豆矮小突变体中差异表达基因［D］.新疆:石河子大学, 2014.（Zhang F, Zhao Y X, Huang X Z. Analysis of differentially expressed genes in soybean dwarf mutants by transcriptome sequencing［D］.Xinjiang:Shihezi University, 2014.）［18］杨楠, 赵凯歌,陈龙清. 蜡梅花转录组数据分析及次生代谢产物合成途径研究［J］.北京林业大学学报,2012,34(S1): 104-107.（Yang N, Zhao K G, Chen L Q.Analysis of transcriptome data and synthesis pathways of secondary metabolites［J］.Journal of Beijing Forestry University,2012, 34(S1): 104-107.）［19］吴凯朝, 黄诚梅, 李杨瑞, 等. Trizol试剂法快速高效提取3种作物不同组织总RNA［J］. 南方农业学报, 2012, 43(12):1934-1939. (Wu K C, Huang C M, Li Y R, et al. Rapid and efficient extraction of total RNA from different tissues of three crops by Trizol reagent［J］. Southern Agricultural Journal, 2012, 43(12): 1934-1939.）［20］Anders S, Huber W. Differential expression analysis for sequence count data［J］. Genome Biology, 2010, 11(10): R106.［21］Kazan K, Manners J M. Linking development to defense: Auxin in plant-pathogen interactions［J］. Trends in Plant Science, 2009, 14(7):373-382.［22］Alazem M, Lin N S. Roles of plant hormones in the regulation of host-virus interactions［J］. Molecular Plant Pathology, 2015, 16(5):529-540.［23］Miguel A L, Bannenberg G, Castresana C. Controlling hormone signaling is a plant and pathogen challenge for growth and survival［J］. Current Opinion in Plant Biology, 2008, 11(4):420-427.［24］Miya A, Albert P, Shinya T, et al. CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis［J］. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(49): 19613-19618.［25］Robert-Seilaniantz A, Grant M, Jones J D G. Hormone crosstalk in plant disease and defense: More than just JASMONATE-SALICYLATE antagonism［J］. Annual Review of Phytopathology, 2011, 49(1):317-343.［26］Thilmony R, Underwood W, He S Y. Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7［J］. The Plant Journal, 2006, 46:34-53.［27］Chen Z. Pseudomonas syringae type III effector AvrRpt2 alters Arabidopsis thaliana auxin physiology［J］. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(50):20131-20136.［28］Wang D, Pajerowska-Mukhtar K, Culler A H, et al. Salicylic acid inhibits pathogen growth in plants through repression of the auxin signaling pathway［J］. Current Biology, 2007, 17(20):1784-1790.［29］Kazan K, Manners J M. Linking development to defense: Auxin in plant-pathogen interactions［J］. Trends in Plant Science, 2009, 14(7):373-382.［30］Ding X, Cao Y, Huang L, et al. Activation of the indole-3-acetic acid-amido synthetase gh3-8 suppresses expansin expression and promotes salicylate- and jasmonate-independent basal immunity in rice［J］. The Plant Cell Online, 2008, 20(1):228-240. ［31］Park J E, Park J Y, Kim Y S, et al. GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis［J］. Journal of Biological Chemistry, 2007, 282(13):10036-10046.［32］Abreu M E, Munne-Bosch S. Salicylic acid deficiency in NahG transgenic lines and sid2mutants increases seed yield in the annual plant Arabidopsis thaliana［J］.Journal of Experimental Botany,2009,60(4): 1261-1271.［33］Wojtaszek P. Oxidative burst: An early plant response to pathogen infection［J］. Biochemical Journal, 1997, 322(3):681-692.

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