|Table of Contents|

Identification and Response to Phytophthora sojae Infections of JAZ Gene Family in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年06期
Page:
868-878
Research Field:
Publishing date:

Info

Title:
Identification and Response to Phytophthora sojae Infections of JAZ Gene Family in Soybean
Author(s):
HUANG Jian-li DENG Su-shuang SHEN Jia-cheng BU Yuan-peng ZHAO Jin-ming GUO Na XING Han
(College of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China)
Keywords:
Soybean JAZ gene family Bioinformatics Phytophthora sojae qRT-PCR
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.06.0868
Abstract:
JAZ (Jasmonate ZIM-domain) protein, a key transcriptional repressor in JA signaling pathway, plays an important role in plant defense. However, there is limited information about this family. In order to study the characteristic and potential function of the JAZ gene family in soybean, this study identified a total of 24 JAZ genes in the soybean genome by bioinformatics, named as GmJAZ1to GmJAZ24. We extensively analyzed intron-exon distribution and protein motif organization, built phylogenetic tree, and analyzed chromosome localization, cis-acting regulatory elements and response to Phytophthora sojae infections for JAZ family in soybean. The results showed that a total of 24 GmJAZs was unevenly distributed on the fourteen chromosomes with maximum number of genes (four JAZs) on chromosome 9. The JAZ family divided into five clusters (C1~C5) including soybean, Arabidopsis and rice in the phylogenetic tree, The JAZ genes of soybean were the most closely related to Arabidopsis. Promoter analysis revealed that most of soybean JAZ genes contained jasmonic acid (JA) and abscisic acid (ABA) responsive cis-acting elements, and some with defense and stress related cis-acting elements. After Phytophthora sojae infections, C4 subgroup members were significantly upregulated, C1 subgroup members were weakly upregulated, while the C2, C3 and C5 subgroup members were downregulated, which indicated that the soybean JAZ genes exhibited divergent expression patterns in response to Phytophthora sojae treatment. The results of the present study provide valuable information for functional characterization of GmJAZ in response to Phytophthora sojae in soybean.

References:

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