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Preliminary Analysis of Polyamine Composition Content in Soybean Germplasm of the Northeast China(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年06期
Page:
856-861
Research Field:
Publishing date:

Info

Title:
Preliminary Analysis of Polyamine Composition Content in Soybean Germplasm of the Northeast China
Author(s):
ZHENG Yu-hong;  MA Ling-ling;  QIU Hong-mei;  FAN Xu-hong;  ZHANG Yun-feng;  MENG Fan-fan;  SUN Xing-miao; WANG Shu-ming
(Soybean Research Institute, Jilin Academy of Agricultural Sciences /National Engineering Research Center for Soybean, Changchun 130033, China)
Keywords:
Soybean;  Germplasm;  Spermidine;  Polyamines;  Phenotypic identification;  Cluster analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.06.0856
Abstract:
In order to analyze the polyamine content of soybean germplasm resources in the Northeast China and the correlation between different polyamine components, this study adopted the National Standard-Determination of Biogenic Amine Content in Food (GB 5009.208-2008) to determine four polyamine components of 78 soybean germplasms in different maturity stages, including cultivated, wild and semi-wild types. The contents of amines, spermine, putrescine and cadaverine were determined. Phenotypic identification and preliminary analysis of polyamines in soybean germplasm were carried out by genetic diversity, correlation, factor and cluster analysis. The results showed that there were abundant genetic variations in the contents of four polyamines in 78 soybean germplasms. The dispersions of putrescine, spermidine and spermine components were higher, and there were more specific contents in 78 soybean germplasms. The genetic diversity indices of putrescine, cadaveric amine, spermidine and spermine contents in cultivation types were 1.09, 1.09, 1.32 and 1.74, respectively, indicating that there were abundant genotypes and genetic variations regulating spermidine and spermine components. Spermidine content ranged from 0.1 to 1.06 mg·kg-1, with a mean value of 0.53 mg·kg-1 and a coefficient of variation of 18.45%. This indicated that there were significant differences in spermidine content among soybean varieties. The content of polyamines in all types of soybean germplasms was relatively the same, but there was no significant difference between the wild and semi-wild soybean germplasms except that the content of cadaverine in wild and semi-wild soybean germplasms was significantly different（P<0.05）. The content of spermidine was positively correlated with that of spermine, the content of putrescine was positively correlated with that of polyamines, and the genetic relationship between spermidine and spermine was close. Principal component analysis showed that putrescine, spermidine and spermine were the representative factors in polyamines. Cluster analysis showed that 66 cultivar germplasms were divided into three groups. Group 2 and 3 contained fewer germplasms, and most of them were early maturity type.

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