|Table of Contents|

Cloning and Characterization of GmNF-YA11 Gene in Soybean
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《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年05期
Page:
719-725
Research Field:
Publishing date:

Info

Title:
Cloning and Characterization of GmNF-YA11 Gene in Soybean
Author(s):
BAI Yu-cui;  WANG Ping;  NI Zhi-yong;  YU Yue-hua
(College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China)
Keywords:
Soybean;  GmNF-YA11;  Cloning;  Transcriptional activation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.05.0719
Abstract:
The NF-Y transcription factor is a ubiquitous transcription factor complex in eukaryotes, recent studies have found that NF-Y plays an important role in plant embryo development, photosynthesis, flowering time regulation, stress response and so on. Therefore, studying NF-Y transcription factors is important to improve soybean resistance. We cloned the GmNF-YA11 gene from soybean in order to preliminarily understand its basic characteristics. The full-length cDNA sequence is 1 553 bp containing open reading frame of 912 bp, encodes a protein of 303 amino acids with a molecular weight of 34.06 kD and pI 9.50. The GmNF-YA11 genome contains five exons and four introns. Phylogenetic tree analysis showed that GmNF-YA11 and GmNF-YA13 were on the same branch. Transcriptional activity analysis showed that GmNF-YA11 transcription factor didn’t have transcriptional activation function. The analysis of tissue specific expression pattern showed that GmNF-YA11 was expressed in all the tissues detected, the highest expression in the seeds and the lowest expression in the flowers.

References:

［1］刘新旗, 涂丛慧, 张连慧, 等.大豆蛋白的营养保健功能研究现状［J］. 北京工商大学学报(自然科学版), 2012, 30(2):1-6. (Liu X Q, Tu C H, Zhang L H, et al. Research status of nutritional and health function of soybean protein［J］. Journal of Beijing Technology and Business University (Social Sciences Edition), 2012, 30(2):1-6.)
［2］周新安.我国大豆生产与科研现状及其发展对策［J］.作物杂志, 2007(6): 1-4. (Zhou X A. Current situation and development strategy of soybean production and scientific research in China［J］. Crops, 2007(6): 1-4.)
［3］杨如萍, 包振贤, 陈光荣, 等.大豆抗旱性研究进展［J］. 作物杂志, 2012(5): 8-12. (Yang R P, Bao Z X, Chen G R, et al. Advances in drought resistance of soybean［J］. Crops, 2012(5): 8 -12.
［4］Albani D, Robert L S. Cloning and characterization of a Brassica napus gene encoding a homologue of the B subunit of a heteromeric CCAAT-binding factor［J］. Gene(Amsterdam), 1995, 167(1-2): 209-213.
［5］Lévesque-Lemay M, Albani D, Aldcorn D, et al. Expression of CCAAT-binding factor antisense transcripts in reproductive tissues affects plant fertility［J］. Plant Cell Reports, 2003, 21(8): 804-808.
［6］Combier J P, Frugier F, de Billy F, et al. Mt HAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by micro RNA169 in Medicago truncatula［J］. Genes Development, 2006, 20(22): 3084-3088.
［7］Ma X, Zhu X, Li C, et al. Overexpression of wheat NF-YA10 gene regulates the salinity stress response in Arabidopsis thaliana［J］. Plant Physiology and Biochemistry, 2015, 86: 34-43.
［8］Li Y J, Fang Y, Fu Y R, et al. NFYA1 is involved in regulation of postgermination growth arrest under salt stress in Arabidopsis［J］. Plos One,2013, 8(4):e61289.
［9］Jones-Rhoades M W, Bartel D P, Bartel B. MicroRNAs and their regulatory roles in plants［J］. Annual Review of Plant Biology, 2006, 57: 19-53.
［10］Zhao B, Ge L, Liang R, et al. Members of miR-169 family are induced by high salinity and transiently inhibit the NF-YA transcription factor［J］. BMC Molecular Biology, 2009, 10: 29.
［11］Zhang X, Zou Z, Gong P, et al. Over-expression of microRNA169 confers enhanced drought tolerance to tomato［J］. Biotechnology Letters, 2011, 33(2): 403-409.
［12］Quach T N, Nguyen H T M, Valliyodan B, et al. Genome-wide expression analysis of soybean NF-Y genes reveals potential function in development and drought response［J］. Molecular Genetics and Genomics, 2014, 290(3): 1095.
［13］Ni Z, Hu Z, Jiang Q, et al. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress［J］. Plant Molecular Biology, 2013, 82(1-2): 113-129.
［14］王涛, 李永光, 王英琪, 等. 大豆GmNFYB1基因功能预测及表达分析［J］. 基因组学与应用生物学, 2015, 34(5): 1034-1039. (Wang T, Li Y G, Wang Y Q, et al. Functional prediction and expression analysis of soybean GmNFYB1 gene［J］. Genomics and Applied Biology, 2015, 34(5): 1034-1039.
［15］李涵哲. 大豆GmNFYB1互作蛋白分析及与干旱相关下游基因分析［D］. 哈尔滨: 东北农业大学, 2016. (Li H Z. Analysis of GmNFYB1 interaction protein and drought-related downstream genes in soybean［D］. Harbin: Northeast Agricultural University, 2016.
［16］Mantovani R. The molecular biology of the CCAAT-binding factor NF-Y［J］. Gene, 1999, 239(1): 15-27.
［17］Mcnabb D S, Tseng K A, Guarente L. The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor［J］. Molecular and Cellular Biology, 1997, 17(12): 7008-7018.
［18］Gusmaroli G, Tonelli C, Mantovani R. Regulation of the CCAAT-Binding NF-Y subunits in Arabidopsis thaliana［J］. Gene, 2001, 264(2): 173-185.
［19］Luger K, Armin W Mder, Richmond R K, et al. Crystal structure of the nucleosome core particle at 2.8 resolution［J］. Nature, 389: 251-260.
［20］Petroni K, Kumimoto R W, Gnesutta N, et al. The promiscuous life of plant nuclera factor Y transcription factors［J］. Plant Cell, 2012, 24(12): 4777-4792.
［21］Siefers N, Dang K K, Kumimoto R W, et al. Tissue-specific expression patterns of Arabidopsis NF-Y transcription factors suggest potential for extensive combinatorial complexity［J］. Plant Physiology, 2008, 149(2): 625-641.
［22］Cao S, Kumimoto R W, Siriwardana C L, et al. Identification and characterization of NF-Y transcription factor families in the monocot model plant Brachypodium distachyon［J］. PLoS One, 2011, 6: e21805.
［23］Stephenson T J, Mcintyre C L, Collet C, et al. Genome-wide identification and expression analysis of the NF-Y family of transcription factors in Triticum aestivum［J］. Plant Molecular Biology, 2007, 65(1-2):77-92.
［24］Kubo T, Serizawa A. Identification, characterization and interaction of HAP family genes in rice［J］. Journal of Receptor Research, 2008, 7(1-4): 279-289.
［25］Mu J, Tan H, Hong S, et al. Arabidopsis transcription factor genes NF-YA1, 5, 6, and 9 play redundant roles in male gametogenesis, embryogenesis, and seed development［J］. Molecular Plant, 2013, 6(1): 188-201.
［26］Nardini M, Gnesutta N, Donati G, et al. Sequence-specific transcription factor NF-Y displays histone-like DNA binding and H2B-like ubiquitination［J］. Cell, 2013, 152(1-2): 132-143.

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Last Update: 2019-09-20