|Table of Contents|

Establishment of Detection System for Three Genetically Modified Soybean Linesidentification(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年05期
Page:
712-718
Research Field:
Publishing date:

Info

Title:
Establishment of Detection System for Three Genetically Modified Soybean Linesidentification
Author(s):
(Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China)
Keywords:
Multitarget real-time PCR;  Genetically modified soybean;  Rapid analysis;  Event-specific
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.05.0712
Abstract:
Genetically modified soybean lines MON87701, MON87708 and MON87769 are the key monitoring targets in soybean import trade in China. In order to provide a highly efficient technical means for the supervision of these transgenic soybean lines, this study established a multitarget real-time polymerase chain reaction(MT-PCR) assay for simultaneous detection of MON87701, MON87708 and MON87769 in one reaction system. We optimized the primers and probes according to the event-specific border sequences of MON87701, MON87708, MON87769 and the endogenous lectin gene sequence in soybean. The primers/probes concentration optimization, specificity, sensitivity and applicability were tested, respectively. Other transgenic soybean, maize, rice, rapeseed, cotton and non-transgenic soybean were used as test samples in this study. The results showed that the MT-PCR method could accurately detect the corresponding target GM soybean components from various complex samples. The absolute limit of detection for MON87701, MON87708 and MON87769 were 43 copies, equivalent to 0.05% (w/w). This method can be used for the rapid identification of corresponding GM soybean lines.

References:

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Last Update: 2019-09-20