|Table of Contents|

Cloning and Analysis of Receptor-like Kinase Gene GmNIK in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年05期
Page:
704-711
Research Field:
Publishing date:

Info

Title:
Cloning and Analysis of Receptor-like Kinase Gene GmNIK in Soybean
Author(s):
（Soybean Research Institute, Nanjing Agriculture University/Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture/National Center for Soybean Improvement/National Key Laboratory for Crop Genetic and Germplasm Enhancement, Nanjing 210095, China）
Keywords:
Soybean;  GmNIK;  SMV;  Bioinformatics;  LRR-RLK;  Expression characteristics
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.05.0704
Abstract:
Soybean mosaic virus (SMV) is one of the major diseases in soybean producing areas of China, and SC18 is an epidemic strain in the southern region. It is important to find the resistance related genes to SC18 strains for improving the resistance of soybean. We have mapped the related segment resistant to SC18 on the 80 kb interval of soybean chromosome 2, and found that there was a receptor-like protein kinase (LRR-RLK) gene GmNIK which contained leucine repeat. To explore the structure and expression characteristics of soybean GmNIK-coding gene, we cloned the GmNIK gene from the resistant variety Kefeng 1 and analyzed the gene with bioinformatics methods. In addition, we analyzed the relative expression of GmNIK in different tissues and different periods of soybean and detected the expression characteristics under SMV induction by qRT-PCR. The results showed that the ORF of GmNIK was 1 866 bp, and encoded a protein of 621 amino acids with a typical LRR-RLK structure. The amino acid sequence of GmNIK was highly homology with the co-receptor of the cloned R genes, and was closely related to the NIK gene in alfalfa and peanut of leguminous plants. The GmNIK promoter region contained defense and stress response element, SA response element and other cis-acting elements, which induced this gene could respond to the infection of SC18. It can be detected that the gene expressed in root hairs, roots, stems and leaves. After infected by SC18, transcription levels of GmNIK in leaves of resistant and susceptible varieties were different, it was predicted that GmNIK was related to the resistance of soybean to SC18 strain. This study lays a foundation for the identification of soybean resistance genes against soybean mosaic virus and the discovery of resistance mechanisms against soybean mosaic virus.

References:

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Last Update: 2019-09-20