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Study on the High Efficiency Culture Method of Growth and Sporulation of Soybean Frogeye Leaf Spot Pathogen(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年04期
Page:
589-596
Research Field:
Publishing date:

Info

Title:
Study on the High Efficiency Culture Method of Growth and Sporulation of Soybean Frogeye Leaf Spot Pathogen
Author(s):
MA Ling-ling1; 2; LIU Nian-xi2; ZHENG Yu-hong2; LI Zhi2; LIU Jia2; YI Zhi-gang2; DONG Zhi-min2; WANG Shu-ming2
（1.College of Agriculture, Jilin Agricultural University, Changchun 130118, China; 2.Soybean Research Institute, Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean, Changchun 130033, China）
Keywords:
Soybean Frogeye leaf spot;  V8 medium;  Sporulation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.04.0589
Abstract:
In order to explore the effective method of mycelium growth and sporulation ofCercospora sojina Hara, we studied the effects of V8 vegetable juice and calcium carbonate content used in V8 culture medium on mycelial growth rate and spore yield, and obtained the optimum composition distribution ratio. Five different concentrations of V8 vegetable juice and five different concentrations of calcium carbonate were used to make up of 25 different components. We used the widely used PDA medium as the control medium to inoculateCercospora sojina Hararace 7 for dark culture. Colony growth and spore production were measured every 2 days and compared with each other. The results showed that colony diameter, growth rate, growth area and spore production of V8 medium in 25 different components were larger than those of PDA medium. The colony diameter of V8 medium grew fastest at 3.47 mm?d-1, the spore yield was up to 9.87×106 per dish. On the fifth day of culture, the pathogens had the greatest growth vigor. On the day 11-13, the sporulation amount reached the peak, which was the most suitable time for sporulation. When the medium combination A4b4 calcium carbonate content was 1.5 g?L-1 and the V8 vegetable juice concentration was 15%, it is most suitable for colony diameter growth, and the colony growth area is the largest. When the medium combination A4b3 calcium carbonate content was 1.5 g?L-1 and the V8 vegetable juice concentration was 12%, the sporulation amount was the highest.

References:

［1］顾鑫,丁俊杰.大豆灰斑病的研究现状［J］.中国农学通报,2010,26(9): 303-306. (Gu X, Ding J J. Research status of soybean frogeye leaf spot［J］. China Agronomy Bulletin, 2010, 26(9): 303-306.)

［2］Zhang G, Bradley C A. Survival ofCercospora sojinaon soybean leaf debris in Illinois［J］. Plant Health Progress, 2014, 10:92-96.
［3］李博宇.黑龙江省大豆品种对灰斑病抗病性丧失原因分析［D］.哈尔滨: 黑龙江大学, 2014. (Li B Y. Analysis on the reason of losing disease resistance of soybean varieties to soybean leaf frogeye spot in Heilongjiang province［D］. Harbin: Heilongjiang University, 2014.)
［4］李沐慧.东北地区大豆田病害调查研究与鉴定［D］.沈阳: 沈阳农业大学, 2016. (Li M H. Investigation and identification of soybean diseases in northeast China［D］. Shenyang: Shenyang Agricultural University, 2016.)
［5］程伟,常芳国,赵团结,等.大豆灰斑病发生特点及抗病遗传育种研究进展［J］.安徽农业科学, 2015, 43(30): 22-25, 50. (Cheng W, Chang F G, Zhao T J, et al. Progress in genetic breeding of soybean frogeye leaf spot and disease resistance ［J］. Anhui Agricultural Sciences, 2015, 43(30): 22-25, 50.)
［6］董志敏,厉志,刘佳,等.大豆抗灰斑病研究进展［J］. 作物杂志, 2017(3): 1-5. (Dong Z M, Li Z, Liu J, et al. Advances in studies on resistance to frogeye leaf spot in soybean［J］. Crop Magazine, 2017(3): 1-5.)
［7］马淑梅.黑龙江省大豆灰斑病研究现状［J］.植物保护, 1992(4): 34-36. (Ma S M. Present situation of soybean frogeye leaf spot in Heilongjiang province［J］. Plant Protection, 1992(4): 34-36.)
〖HJ1.35mm〗［8］Juliane N C, Valéria C G, Erlei M R, et al. The combined effects of temperature and leaf wetness periods on soybean frogeye leaf spot intensity［J］. Semina:Ciências Agrárias, 2016, 37(1): 77-84.
［9］迟晶.大豆灰斑病致病性变异与品种抗性分析［D］. 哈尔滨: 黑龙江大学, 2012. (Chi J. Delayed crystal analysis of pathogenicity variation and variety resistance of soybean frogeye leaf spot［D］. Harbin: Heilongjiang University, 2012.)
［10］顾鑫,丁俊杰,杨晓贺,等. 2008-2009年黑龙江省大豆灰斑病生理小种的监测［J］.大豆科学,2010,29(3): 540-542. (Gu X, Ding J J, Yang X H, et al. (Monitoring of physiological races of soybean frogeye leaf spot in Heilongjiang province from 2008 to 2009［J］. Soybean Science, 2010, 29(3): 540-542.)
［11］Muller M F, Barnes I, Kunene N T, et al. Cercospora zeina from maize in South Africa exhibits high genetic diversity and lack of regional population differentiation［J］. Phytopathology, 2016, 106(10): 1194-1205.
［12］廖林.大豆灰斑病研究概况及展望［J］. 中国农学通报,1992(1): 6-9. (Liao L. General situation and prospect of soybean frogeye leaf spot research［J］. China Agricultural Science Bulletin, 1992(1): 6-9.)
［13］刘亚光,王继才,杨庆凯.大豆灰斑病菌(Cercospora sojina Hara)毒素的初步研究［J］.大豆科学, 1999, 18(4): 306-311. (Liu Y G, Wang J C, Yang Q K. Preliminary study on (Cercospora sojina Hara) toxin of soybean frogeye leaf spot［J］. Soybean Science, 1999, 18(4): 306-311.)
［14］丁俊杰,顾鑫,杨晓贺,等.黑龙江省大豆新品系抗灰斑病鉴定［J］.东北农业大学学报,2011,42(10): 120-124. (Ding J J, Gu X, Yang X H, et al. Identification of resistance to frogeye leaf spot of soybean new strain in Heilongjiang province ［J］. Journal of Northeast Agricultural University, 2011, 42(10): 120-124.)
［15］陈思文.玉米灰斑病病菌生理分化及品种抗性研究［D］.沈阳: 沈阳农业大学, 2017. (Chen S W. Studies on physiological differentiation and resistance ofCercosporazeae-maydispathogen［D］. Shenyang: Shenyang Agricultural University, 2017.)
［16］Nwanosike M R O, Mabagala R B. Assessment of maize (Zea maysL.)Exserohium Turcicum(Pass.) Leonard and Sugg. isolates on different culture media in Tanzania［J］. World Journal of Agricultural Research, 2017, 5(6): 292-298.
［17］Alves S,Linares C E, de Loreto E S, et al. Utilization of tomato juice agar(V8 agar) in the presumptive identification of Candida dubliniensis［J］. Revista da Sociedade Brasileira de Medicina Tropica, 2006, 39(1): 92-93.
［18］宋淑云, 郭文广, 张伟, 等. 适宜大豆灰斑病菌生长和产孢的培养基筛选试验［J］.吉林农业科学, 2002(S1): 25-26. (Song S Y, Guo W G, Zhang W, et al. Screening experiment of suitable medium for growth and sporulation of soybean frogeye leaf spot［J］. Jilin Agricultural Science, 2002(S1): 25-26.)
［19］Ji S K, Young S L, Sung K K,et al. Differential responses of soybean cultivars toCercospora sojinaisolates, the causal agent of frogeye leaf spot in Korea［J］. Journal of General Plant Pahtology, 2011, 27(2): 183-186.
［20］Beckman P M, Payne G A. Cultural techniques and conditions influencing growth and sporulation ofCercosporazeae-maydisand lesion development in cornn［J］. Phytopathology, 1983, 73(2): 286-289.
［21］佟淑杰,徐秀德,董怀玉,等.玉米灰斑病菌产孢特性的初步研究［J］.杂粮作物,2005(3):201-203. (Tong S J, Xu X D, Dong H Y, et al. A preliminary study on the sporulation characteristics ofCercosporazeae-maydispathogen［J］. Grain crops,2005(3): 201-203.)

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Last Update: 2019-07-25