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Title:: Sequencing Analysis of Transcriptome During the Different Developmental Stages in Soybean Seed

Author(s):: CHEN Jin-ling; XU Yuan; CHEN Yu-mei; LI Lu-lu; LI Hui-min; QIN Xin-min; （College of Life Science, Guangxi Normal University, Guilin 541004,China）

Keywords:: Soybean; Differential expressed genes; Lipid synthesis; Transcriptome sequencing

PACS:: -

DOI:: 10.11861/j.issn.1000-9841.2019.04.0533

Abstract:: In order to study the mechanism of oil synthesis and accumulation in soybean seeds at different developmental stages at the molecular level, the soybean seeds of 20 d (DD_20), 30 d (DD_30), 40 d (DD_40) and 50 d (DD_50) were used as test materials in transcriptome sequencing. Through the analysis of transcriptome sequencing data, 461 566 988 raw reads were obtained. After filtration, the clean reads of DD_20, DD_30, DD_40 and DD_50 were obtained respectively, as 107 548 920, 111 670 776, 109 339 672 and 108 884 270. The total differential expression genes of DD_20/DD_30, DD_30/DD_40, DD_40/DD_50 were 4 759, 6 245 and 13 763 respectively, of which up-regulated genes were 1 801, 2 941 and 5 695 respectively. KEGG pathway analysis of differential expression genes obtained 134, 133 and 136 KEGG pathways, screened 8 differential expression genes related to lipid synthesis, as ACC, FATB, GPAT, DGAT1, G3PDH, KASI, SAD and FAD2. The results provide an important reference for further study on the regulation mechanism of soybean seed lipid synthesis, as well as for the breeding of high oil content soybean varieties.

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Sequencing Analysis of Transcriptome During the Different Developmental Stages in Soybean Seed(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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