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Cloning of GmbZIP33 Promoter from Soybean and Its Transient Expression Analysis inArabidopsis thaliana(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年04期
Page:
511-516
Research Field:
Publishing date:

Info

Title:
Cloning of GmbZIP33 Promoter from Soybean and Its Transient Expression Analysis inArabidopsis thaliana
Author(s):
BAI Li-juan1; 2;  LIU Wei3;  WANG Zhi-li4;  WANG Wen-ting1; 2;  WU Cun-xiang2; FENG Yong-jun1

(1.School of Life Science, Beijing Institute of Technology, Beijing 100081, China; 2.Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China; 3.Crop Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China； 4.School of Life Science，The Chinese University of Hong Kong, Hong Kong 999077, China)
Keywords:
Soybean; Arabidopsis thaliana;  GUS;  Vascular tissue specific promoter
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.04.0511
Abstract:

Promoter, as an important element in regulating gene expression, determines the temporal, spatial and intensity characteristics of the downstream gene expression. To study the promoter function of soybean GmbZIP33 gene, we cloned 2 316 bp upstream region of GmbZIP33 CDS as the promoter by N-PCR(nested PCR) technique on the basis of previous cloned GmbZIP33 gene sequence, and predicted its cis-acting elements by using PlantCARE online analysis software. It was found that besides the core regulatory elements of TATA-box and CAAT-box, there were also stress resistance response, light response, hormone response, sucrose response elements and tissue-specific expression elements. A vector to regulate the expression of reporter geneGUSwas constructed and transformed intoArabidopsis thalianabyAgrobacterium-mediated floral-dip method to study the expression characteristics of GmbZIP33 promoter. Histochemical staining of transgenicArabidopsisshowed thatGUSgene driven by this promoter was specifically expressed in vascular bundles of different tissues (rosette leaf, flower, pod and root). Real-time fluorescence quantitative PCR of theGUSgene in these four tissues of transgenicArabidopsisshowed that it was most expressed in flower, followed by in pod, suggesting that GmbZIP33 gene might be regulated by many factors and sucrose, and showing the possibility of roles in flower and pod development regulation.

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Last Update: 2019-07-25