|Table of Contents|

Analysis of 7S and 11S Globulin and Their Subunits Relative Content of Soybean Germplasm in Jiangxi Province(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年04期
Page:
503-510
Research Field:
Publishing date:

Info

Title:
Analysis of 7S and 11S Globulin and Their Subunits Relative Content of Soybean Germplasm in Jiangxi Province
Author(s):
ZHAO Xian-wei1 ZHAO Chao-sen1 WANG Rui-zhen1 GUO Bing-fu1 WANG Rong-rong2 CAO Xiao-xin3 ZHOU Jin-gou4
(1.Crops Research Institute of Jiangxi Academy of Agricultural Sciences/Nanchang Branch of National Center of Oilcrops Improvement/Jiangxi Province Key Laboratory of Oilcrops Biology, Nanchang 330200, China; 2.Administration of seeds of Ruichang City, Ruichang 332200, China; 3.Agricultural Technology Extension Station of Liufang Town, Jiujiang 332517, China; 4.Agricultural Technology Extension Station of Shuinan Town, Ji′an 343000, China)
Keywords:
Soybean Germplasm 7S globulin l1S globulin Subunit
PACS:
-
DOI:
DOI:10.11861/j.issn.1000-9841.2019.04.0503
Abstract:
In order to clarify the relative content variation of globulin and their subunits, and then screening excellent germplasm to improve the nutritional and processing quality of soybean. This study analyzed 7S and 11S globulin and subunits content of 131 soybean accessions originated from Jiangxi province by SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The study showed that the variation range, average and variable coefficient of relative content of 7S and 11S globulins were 18.01%-52.21%, 32.64%, 19.46% and 47.79%-81.99%, 67.35% and 9.43%, respectively. The variation of 11S/7S ration ranged from 0.92 to 4.55, and the average and variable coefficient of 11S/7S were 2.19 and 30.64%, respectively. The gathered data implied that soybean germplasm of Jiangxi province contains abundant genetic diversity of relative contents of 7S, 11S globulin and their subunits, among which 11S/7S ratios of 3 germplasm were higher than 4.0, and 4 accessions showed relative lower contents ofα′, αandβsubunits than others. This study provides a useful reference to improve high-quality breeding of soybean.

References:

[1]王磊, 王慧中, 藕冉, 等. 大豆主要贮藏蛋白组分遗传改良研究进展[J]. 中国油料作物学报, 2018, 40(4): 608-612. (Wang L, Wang H Z, Ou R, et al. Recent advances in genetic improvement of soybean seed main storage proteins[J]. Chinese Journal of Oil Crop Sciences, 2018, 40(4): 608-612.)

[2]Krishnan H B,Jiang G,Krishnan A H,et al. Seed storage protein composition of non-nodulating soybean [Glycine max(L.) Merr.] and its influence on protein quality[J]. Plant Science,2000,157:191-199.
[3]郭顺堂, 孟岩, 张雪梅, 等. 中国大豆蛋白亚基构成分析与缺失部分亚基的特异大豆品种的筛选[J]. 作物学报, 2006, 32(8): 1130-1134. (Guo S T, Meng Y, Zhang X M, et al. Analysis of protein subunit composition of Chinses soybean [Glycine max(L.) Merrill] cultivars and screening of soybean cultivars lacking some subunits[J]. Acta Agronomica Sinica, 2006, 32(8): 1130-1134.)
[4]Koshiyama I. Chemical and physical properties of a 7S protein in soybean globulins [J]. Cereal Chemistry, 1968, 45: 394-404.
[5]Staswick P E, Hermodson M A, Nielsen N C. Identification of the acidic and basic subunit complexes of glycinin[J]. Journal of Biological Chemistry, 1981, 256 (16): 8752-8755.
[6]王绍东, 李远明, 刘伟, 等. 聚丙烯酰胺凝胶电泳快速检测大豆过敏原蛋白Gly m Bd 30K 缺失方法[J]. 大豆科学, 2011, 30(4): 638-642. (Wang S D, Li Y M, Liu W, et al. A fast detection method for screening of Gly m Bd 30K allergen protein using sodium dodecyl sulfate polyacrylamide gel electrophoresis in soybean seeds[J]. Soybean Sciences, 2011, 30(4): 638-642.)
[7]Ogawa T, Bando N, Tsuji H, et al. Investigation of the lgE-binding proteins in soybeans by immunoblotting with the sera of the soybean-sensitive patients with the atopic dermatitis[J]. Ournal of Nutritional Sciences and Vitaminology, 1991, 37(6): 555-565.
[8]Ogawa T, Samoto M, Takahashi K. Soybean allergens and hypoallergenic soybean products[J]. Journal of Nutritional Sciences and Vitaminology, 2000, 46(6): 271-279.
[9]Samoto M, Fukuda Y, Takahashi K, et al. Substantially complete removal of three major allergenic soybean proteins (Gly m Bd 30K, Gly m Bd 28K, and theα-subunit ofβ-conglycinin) from soy protein by using a mutant soybean, Tohoku 124[J]. Bioscience Biotechnology and Biochemistry, 1997, 61(12): 2148-2150.
[10]张明俊, 李忠峰, 于莉莉, 等. 大豆籽粒蛋白亚基变异种质的鉴定与筛选[J]. 作物杂志, 2018(3): 44-50. (Zhang M J, Li Z F, Yu L L, et al. Identification and screening of protein subunit variation germplasm from both mutants and natural population in soybean[J]. Crops, 2018(3): 44-50.)
[11]刘珊珊, 滕卫丽, 姜自芹, 等. 大豆7S球蛋白α-亚基缺失型种质创新[J]. 作物学报, 2010, 36 (8): 1409-1413. (Liu S S, Teng W L, Jiang Z Q, et al. Development of soybean germplasm lacking of 7S globulinα-subunit[J]. Acta Agronomica Sinica, 2010, 36(8): 1409-1413.)
[12]宋波, 蓝岚, 田福东, 等. 大豆7S球蛋白α′亚基缺失及(α′+α)亚基双缺失品系的回交转育[J]. 作物学报, 2012, 38(12): 2297-2305. (Song B, Lan L, Tian F D, et al. Development of soybean lines withα′-subunit or (α′+α) subunits deficiency in 7S globulin by backcrossing[J]. Acta Agronomica Sinica, 2012, 38(12): 2297.)
[13]拓云, 霍彩琴, 田福东, 等. SSR标记辅助回交转育大豆7S球蛋白α-亚基致敏蛋白缺失新品系[J]. 中国油料作物学报, 2014, 36(1): 1-9. (Tuo Y, Huo C Q, Tian F D, et al. Soybean 7Sα-subunit deficiency lines developed by backcrossing assisted by SSR marker background selection [J]. Chinese Journal of Oil Crop Sciences, 2014, 36(1): 1-9.)
[14]张国敏, 张亚琴, 舒英杰, 等. 三种大豆种子贮藏蛋白亚基缺失种质的筛选与鉴定[J]. 大豆科学, 2015, 34(1): 21-31. (Zhang G M, Zhang Y Q, Shu Y J, et al. Screening and identification of three types of soybean lines lacking different seed storage protein subunits[J]. Soybean Sciences, 2015, 34(1): 21-31.)
[15]国博闻, 赵雪, 魏小双, 等. 具有中国大豆遗传背景的7S与11S多亚基缺失型大豆新品系的创制[J]. 作物杂志, 2016(2): 43-49. (Guo B W, Zhao X, Wei X S, et al. Development of set of 7S and 11S multi-subunit-deficient mutants with Chinese soybean genetic background[J]. Crops, 2016(2): 43-49.)
[16]宋波. 大豆7S致敏蛋白α-亚基缺失型近等基因系的近等性评价与应用[D]. 哈尔滨: 东北农业大学, 2017: 10-25. (Song B. Evaluation and application of near-isogenic lines for allergenicα-subunit deficiency of 7S globulin in soybean (Glycine maxL. Merrill) [D]. Harbin: Northeast Agricultural University, 2017: 10-25.)
[17]Patil G, Mian R, Vuong T, et al. Molecular mapping and genomics of soybean seed protein: A review and perspective for the future[J]. Theoretical and Applied Genetics, 2017, 130: 1975-1991.
[18]王文真, 刘兴媛, 曹永生, 等. 中国大豆种质资源的蛋白质含量研究[J]. 作物品种资源, 1988(1): 35-36. (Wang W Z, Liu X Y, Cao Y S, et al. Protein content analysis of Chinese soybean germplasm resources [J]. Crop Varieties Resources, 1988(1): 35-36.)
[19]姜莹, 吴娴静, 董德坤, 等. 浙江省大豆种质资源蛋白亚基构成分析[J]. 浙江农业学报, 2010, 22(4): 403-407. (Jiang Y, Wu X J, Dong D K, et al. Analysis on protein subunit contents of soybean germplasm in Zhejiang province[J]. Acta Agriculturae Zhejiangensis, 2010, 22(4): 403-407.)
[20]王林林, 关荣霞, 齐峥, 等. 大豆微核心种质与育成品种的种子蛋白11S/7S比值的分析[J]. 植物遗传资源学报, 2008, 9 (1): 68-72. (Wang L L, Guan R X, Qi Z, et al. Analysis of 11S/7S ratio core collection between soybean mini and cultivars[J]. Journal of Plant Genetic Resources, 2008, 9(1): 68-72.)
[21]卢为国, 王树峰, 李卫东, 等. 大豆籽粒贮藏蛋白11S/7S比值与生态因子相关关系的研究[J]. 中国农业科学, 2005, 38(5): 1059-1064.(Lu W G, Wang S F, Li W D, et al. Efects of eco-physiological factors on storage protein 11S/7S ratio in soybean seeds[J]. Scientia Agricultura Sinica, 2005, 38(5): 1059-1064.)

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Last Update: 2019-07-25