|Table of Contents|

Bioinformatics Analysis of Gene GmGW2 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年03期
Page:
379-384
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis of Gene GmGW2 in Soybean
Author(s):
(1.College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China; 2.College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China; 3.Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs of the People′s Republic of China, Beijing 102206, China)
Keywords:
Soybean;  Zhonghuang 10;  GmGW2;  Bioinformatics analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.03.0379
Abstract:
The GW2 gene in diverse plants can control grain width and grain weight. In order to predicet and analysis the function of GW2 gene in soybean, the cDNA sequence of the GmGW2 gene was successfully cloned from soybean by the method of homologous cloning. In order to continue the in-depth study, the full-length DNA sequence of GmGW2 gene was cloned from soybean Zhonghuang 10 and detailed bioinformatics analysis was carried out in this experiment. The results showed that the cloned GmGW2 was consisted of 8 exons and 7 introns, encoding 431 amino acids, which was predicted to be located in the nucleus or cytoplasm, without transmembrane domain and signal peptide, and it was an unstable hydrophilic protein with a Zinc finger, RING-type domain. The secondary and third structure prediction analysis showed that α-helix and irregular curl were the main structural elements in the overall protein structure. Phylogenetic tree showed that the gene was closely related to Cajanus cajan, Abrus precatorius and Cicer arietinum. GmGW2 gene may also play a regulational function in soybean seed traits above the results. The results of this study laid the foundation for further digging and verifying the function of GmGW2 gene, aiming to provide useful information for soybean yield breeding.

References:

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Last Update: 2019-05-30