|Table of Contents|

Bioinformatic Analysis of NRT1.2 Homologous Gene in Soybean
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《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年03期
Page:
371-378
Research Field:
Publishing date:

Info

Title:
Bioinformatic Analysis of NRT1.2 Homologous Gene in Soybean
Author(s):
ZHU Lin;  ZUO Yan-yan;  CAO Jin-shan;  WANG Xiao-di;  YANG Wei;  WANG You-ning
(College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
Keywords:
Soybean;  Nitrogen nutrient;  NRT1.2;  Symbiotic nitrogen fixation;  Bioinformatics
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.03.0371
Abstract:
AtNRT1.2 functions as a low affinity nitrate transporter involved in nitrae uptake in Arabidopsis. Based on studying the homologous genes of AtNRT1.2 in soybean and identifying putative genes involved in the regulation of symbiotic nitrogen fixation in soybean, it will provide evidence to uncover its’ biological function. In this study, the homologous genes of AtNRT1.2 have been analyzed using different bioinformatic softwares and methods. It was found that GmNRT1.2a, GmNRT1.2b, GmNRT1.2c and GmNRT1.2d showed to have higher similarity with the homologous genes in Phaseolus vulgaris and Medicago truncatula. In addition, the greater the similarity between the amino acid sequences of GmNRT1.2a and GmNRT1.2b were found and showed the closer relationship between them compared with other homologous genes. Similar to AtNRT1.2, all of GmNRT1.2s contained the conserved transmembrane structure. Based on the expression data collected from SoyBase, GmNRT1.2a and GmNRT1.2b appeared to be highly expressed in roots and nodules of soybean. In summary, our results provide clues for the role of GmNRT1.2s in nitrate absorption and symbiotic nitrogen fixation of soybean.

References:

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Last Update: 2019-05-30