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Isolation, Identification and Control Effect of Biocontrol Bacteria Against Soybean Fusarium Oxysporum Disease(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年02期
Page:
274-279
Research Field:
Publishing date:

Info

Title:
Isolation, Identification and Control Effect of Biocontrol Bacteria Against Soybean Fusarium Oxysporum Disease
Author(s):
(School of Energy and Environment Science, Yunnan Normal University, Kunming 650500, China)
Keywords:
Soybean;  Biological control;  Fusarium oxysporum;  Plant protection
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.02.0274
Abstract:
Abstract: In order to solve the loss of soybean planting caused by Fusarium oxysporum disease and avoid the risk of chemical agents to the quality of agricultural products and human health, the biocontrol bacteria against soybean F.oxysporum were isolated by microbial method, and its type were identified by molecular biology and bioinformatics. The control effect of biocontrol bacteria against soybean F.oxysporum was tested by greenhouse experiments. A total of 144 strains were isolated, strain Y8 had strong control effect on F.oxysporum soybean specific type. The strain was identified as Pseudomonas. The greenhouse experiment results showed that Y8 strain could effectively protect soybean from F.oxysporum infection. Compared with control treatment, the total and ground biomass of soybean were 45.9% and 73.3% higher, produced 35.4% and 22.7% more soybean seeds and chlorophyll content. This might be due to the successful colonization of strain Y8 in soybean rhizosphere, which inhibited the growth of F.oxysporum, and its secondary metabolites could promote the growth of soybean. The Pseudomonas microorganisms found in this study could provide an effective way to control soybean F.oxysporum disease.

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Last Update: 2019-04-01