|Table of Contents|

Screening and Identification of a Wild Soybean Endophytic Fungus YD-25 and Its Anti-heavy Metal Activity(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年02期
Page:
258-266
Research Field:
Publishing date:

Info

Title:
Screening and Identification of a Wild Soybean Endophytic Fungus YD-25 and Its Anti-heavy Metal Activity
Author(s):
XU Meng1;  WANG Yu-nan1;  LI Yan-jing1;  LI Meng-xue1;  WANG Lan-lan1; YU Cui-mei2;  MA Lian-ju1
(1.College of Life Science, Shenyang Normal University, Shenyang 110034, China; 2.Shenyang Agricultural University, Shenyang 110866, China)
Keywords:
Glycine soja;  Endophytic fungus;  Promoting growth;  Heavy metal tolerance
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.02.0258
Abstract:
In order to enrich the endophytic fungi resources of wild soybeans and explore the biological activities of endophytic fungi of wild soybeans. An endophytic fungus strain YD-25 was isolated from Glycine soja plants and identified by combining morphology with molecular biology. The optimum growth conditions of the strain were determined by controlling single factor variable experiment and orthogonal experiment. The growth promoting effect of the strain was determined by comparing the plant height of the soybean seedlings in the intracellular, extracellular, and control groups. The ability of the strain to resist heavy metals such as copper, lead and cadmium and the enzyme activity of the cells under heavy metal stress were determined. The results showed that the YD-25 colony was white at the beginning, and later turned into orange, the mycelium branched and sporulated. The strain was identified as Epicoccum nigrum. The optimal pH of the strain was 3.0, and the optimized culture conditions were soluble starch 4%, yeast dip powder 0.5%, KH2PO4 0.15%, MgSO4 0.05%, and cultured for 8 d. The extracellular product of YD-25 strain had a good growth promoting effect on soybean seedlings, and the growth promoting rates on 4th and 6th day reached 15.4% and 18.1%, respectively. At the same time, it had good resistance to heavy metal cadmium, lead and copper. Except for the SOD activity of Cd2+ stressed YD-25 strain, the enzyme activities of YD-25 strains under other heavy metal stresses were increased in different degree with the increase of heavy metal concentration.

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Last Update: 2019-04-01