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Identification and Expression Analysis of the Whirly Gene Family in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年02期
Page:
204-211
Research Field:
Publishing date:

Info

Title:
Identification and Expression Analysis of the Whirly Gene Family in Soybean
Author(s):
LI Qing1; 2; WANG Hong1; GUO Lu-qin1; 3; LUO Qiu-lan1; 4
(1.College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, China; 2.College of Optoelectronic Engineering/Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China; 3.College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China; 4.College of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou 521000, China)
Keywords:
Soybean;  Whirly gene family;  Gene expression;  Drought;  Salt stress
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.02.0204
Abstract:
Whirly family is a kind of plant-specific transcription factor, which can bind to single-stranded DNA and play myriad roles both in the nucleus and organelles. However, no research on the functions of the Whirly genes in soybean has been reported. To provide a theoretical basis for further functional study of soybean Whirly genes, the genome-wide identification and comprehensive description of this family were firstly carried out in this study. In total, 7 Whirly genes were identified in soybean, and then their physical and chemical properties, subcellular localizations, evolutionary relationships, gene structures, motif compositions, three-dimensional structures and gene expression patterns were comprehensively analyzed. The 7 GmWHY genes were divided into two clades based on their phylogenetic relationships, gene structures and the motif compositions. Protein three-dimensional structure analysis showed that GmWHY5 lacked β1-4 and α1 due to the absence of N-terminal, which was quite different from other soybean Whirly proteins. We further studied the expression profiles of GmWHY genes using the released transcriptome data, and found their tissular and stressed expression patterns varied a lot. For example, the expression levels of GmWHY1, GmWHY2, GmWHY6 and GmWHY7 were significantly higher than those of GmWHY3, GmWHY4 and GmWHY5. Together, these findings will provide a valuable resource for characterizing the GmWHY gene family and lay a foundation for further elucidation of their molecular mechanisms.

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Last Update: 2019-04-01