|Table of Contents|

Genetic Diversity Analysis of Wild Soybean (Glycine soja) in Shanxi Province Based on SSR Analysis(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年02期
Page:
189-197
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Wild Soybean (Glycine soja) in Shanxi Province Based on SSR Analysis
Author(s):
ZHANG Hai-ping;  CHEN Yan;  WANG Zhi;  WANG Hai-gang;  ZHOU Jian-ping
(Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement, Taiyuan 030031, China)
Keywords:
Shanxi;  Wild soybean;  Gene diversity;  Genetic structure;  Cluster analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.02.0189
Abstract:
Understanding of the genetic diversity of wild soybean resources from Shanxi province is a basis research on its origin and evolution, it will provide a theoretical basis for the exploitation of excellent germplasm and the efficient utilization of wild soybean resources. In this study, genetic diversity of wild soybean resources from 32 counties belong to 9 regions in Shanxi province was analyzed using 52 simple sequence repeat(SSR) markers. A total of 450 alleles were detected with 8.7 alleles per locus ranging from 3 to 19. The major allele frequency ranged 0.185 7-0.885 7 with the mean value of 0.421 0. The gene diversity index ranged 0.210 2-0.871 8 with the mean value of 0.710 0. The polymorphism information content ranged 0.201 9-0.858 4 with the mean value of 0.679 7. According to geographical distribution, all materials were divided into three groups-North, Middle and South. Genetic diversity of every group was evaluated. The allele number, the average gene diversity index and the average polymorphism information content of the middle resources were the highest, the north resources followed, the south resources the lowest. The results of average major allele frequency of three groups were contrary to the results of the allele number. Experimental materials were divided into three groups based on genetic structure and genetic distance respectively. Results of two division methods were similarity. GroupⅠwas composed of part of middle resources, groupⅡwas composed of another part of middle resources and northern resources, group Ⅲ was composed of southern resources. It was found that cluster results were approximately consistent with the geographical distribution of the wild soybean. Wild soybean resources in Shanxi province had rich variation. Middle resources had the highest gene diversity, northern resource the next highest and southern resources the lowest. Therefore, it is speculated that introduction and domestication is occurred between the North and the Middle of Shanxi. The middle region is the center of genetic diversity of wild soybean in Shanxi province.

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Last Update: 2019-04-01