References:
［1］James C. Global status of commercialized Biotech/GM crops:2009［M］.New York：The International Service for the Acquisition of Agri-biotech Applications(ISAAA),2009.
［2］王南,李海燕. 转基因大豆概况及其安全性［J］.吉林农业, 2016(19):118. (Wang N, Li H Y. General situation and safety of transgenic soybean［J］. Jilin Agriculture, 2016(19):118.)
［3］赵必迁. 大豆黄酮在产蛋鸡生产上应用的研究进展［J］.广东饲料,2017,26(8):31-33. (Zhao B Q. Research progress on application of daidzein in laying hens［J］. Guangdong Feed, 2017,26(8):31-33.)
［4］时玉强,鲁绪强,马军,等. 大豆蛋白在传统豆制品中的应用［J］.中国油脂,2017,42(3):155-158. (Shi Y Q, Lu X Q, Ma J, et al. Application of soybean protein in traditional bean products［J］. China Oils and Fats,2017,42(3):155-158.)
［5］李玉珍,肖怀秋,兰立新. 大豆分离蛋白功能特性及其在食品工业中的应用［J］.中国食品添加剂,2008(1):121-124. (Li Y Z, Xiao H Q, Lan L X. Functional characterizations of SPI and its applications in food industry［J］. China Food Additives,2008(1):121-124.)
［6］李景. 大豆磷脂的功能特性及应用现状［J］.安徽农学通报,2017,23(22):102-104.(Li J.The Function and application of soybean phospholipid［J］. Anhui Agricultural Science Bulletin,2017,23 (22):102-104.)
［7］李彦磊.改性大豆分离蛋白可生物降解材料的研究［D］.洛阳:河南工业大学,2014.(Li Y L, Study on biodegradable materials of modified soy protein isolate［D］.Luoyang: Henan University of Technology,2014.)
［8］阮金丽,程晨,陈丽华,等.深圳市居民对转基因食品及其标识认知的再调查与分析［J］.现代食品科技,2013,29(4):848-852，871.（Ruan J L, Cheng C, Chen L H, et al. Investigation and analysis of consumer recognition of genetically modifed foods and transgenic labelling -a case study of Shenzhen city［J］ . Modern Food Science and Technology,2013,29(4):848-852，871.）
［9］马博涵. 市售转基因食品的调查［J］.中国战略新兴产业,2017(48):1-3.(Ma B H. Survey of genetically modified foods sold in market［J］. China Strategic Emerging Industry,2017(48):1-3.)
［10］ 马建荣,余永红.市场销售大豆的转基因检测［J］.广东化工,2018(2):37-38. (Ma J R, Yu Y H. Transgenic detection of several commercial soybean［J］. Guangdong Chemical Industry, 2018(2):37-38.)
［11］黄靖,何敏恒,许喜林. 广州市售豆制品转基因成分的检测与分析［J］.中国酿造,2014, 33(9):138-142. (Huang J, He M H, Xu X L. Detection and analysis of genetically modified ingredients in soybean products in Guangzhou［J］. China Brewing,2014, 33(9):138-142.)
［12］杨永存,杨冬燕. 2012-2015年深圳市售转基因大豆及其产品的监测结果分析［J］.食品安全质量检测学报,2016,7(9):3092-3097.(Yang Y C, Yang D Y. Monitoring results of genetically modified soybean and its products from Shenzhen market in 2012-2015［J］. Journal of Food Safety and Quality,2016,7(9):3092-3097.)
［13］袁建琴,赵江河,史宗勇,等. 动物饲料中转基因抗草甘膦大豆GTS40-3-2成分的检测［J］.大豆科学,2016,35(2):295-300.(Yuan J Q, Zhao J H, Shi Z Y, et al. The detection of transgenic soybean GTS 4032 component in animal feed［J］. Soybean Science,2016,35(2):295-300.)
［14］陈颖,王媛,徐宝梁. 食品加工工艺对大豆内源基因降解变化规律的影响［J］.中国粮油学报,2005,20(4):60-64.(Chen Y, Wang Y, Xu B L. Effect of food processing technology on the variation of endogenous gene degradation in soybean［J］. Journal of the Chinese Cereals and Oils Association,2005,20(4):60-64.)
［15］郑文杰,刘烜,刘伟,等. 转基因大豆加工产品的定性PCR 检测［J］.农业生物技术学报,2003,11(5): 467-471. (Zheng W J, Liu H, Liu W, et al. Qualitative analysis of the processed genetically modified soybean products by PCR-based method［J］. Journal of Agricultural Biotechnology,2003,11(5): 467-471.)
［16］Debode F, Janssen E, Berben G. Physical degradation of genomic DNA of soybean flours does not impair relative quantification of its transgenic content［J］. European Food Research and Technology, 2007, 226(1): 273-280.
［17］Bauer T, Weller P, Hammes W P, et al. The effect of processing parameters on DNA degradation in food［J］. European Food Research and Technology, 2003, 217(4): 338-343.
［18］董立明,李葱葱,邢珍娟,等. 利用多重PCR技术快速检测五个转基因大豆品系［J］.大豆科学,2016,35 (6):1002-1007.(Dong L M, Li C C, Xing Z J, et al. Rapid detection of five genetically modified soybean lines by multiplex PCR method［J］. Soybean Science, 2016,35 (6):1002-1007.)
［19］王凤军,叶素丹,包永华. 转基因大豆及其制品二重荧光定量 PCR 的建立与验证［J］.食品工业科技,2018(8):236-239.(Wang F J, Ye S D, Bao Y H. Establishment and verified of a duplex fluorescence quantitative PCR for screening of genetically modified soybeans and products［J］. Science and Technology of Food Industry,2018(8):236-239.)
［20］GB/T 35535-2017.大豆、油菜中外源基因成分的测定膜芯片法［S］.北京:全国生化检测标准化技术委员会,2017.(GB/T 35535-2017. Detection of genetically modified components in soybean and canola membrane-based gene-chip method［S］.Beijing: National Technical Committee on Biochemical Testing of Standardization Administration，2017.)
［21］国家质量监督检验检疫总局. 常见食品过敏原可视芯片检测方法:SN/T 4417-2016［S］.北京:全国信息与文献标准化技术委员会,2016.(General Administration of Quality Supervision, Inspection and Quarantine. Food allergen detection with visual biosensor chips:SN/T 4417-2016［S］.Beijing: China National Information Technology Standardization Network,2016.)
［22］王永,兰青阔,朱珠,等. 转基因大豆GTS40-3-2成分现场可视化检测方法的建立［J］.大豆科学,2014, 33(4): 570-573.(Wang Y, Lan Q K, Zhu Z, et al. Development of visual loop-mediate isothermal amplification assay for herbicide-resistant soybean GTS40-3-2 and its derivates［J］. Soybean Science, 2014, 33(4):570-573.)
［23］Vogelstein B, Kinzler K W.Digital PCR［J］.Proceedings of the National Academy of Sciences of the United States of America,1999,96(16):9236-9241.
［24］李春勇. 数字PCR技术原理及应用［J］.生物技术世界,2014(10):10-13.(Li C Y. Principle and application of digital PCR technology［J］.Biotech world,2014(10):10-13.)
［25］于晓帆,高宏伟,孙敏,等．荧光PCR和数字PCR法检测转基因DAS-44406-6品系大豆［J］.食品科学,2016,37(16) :235-241.(Yu X F, Gao H W, Sun M, et al. Detection of genetically modified soybean event DAS-44406-6 by real-time PCR method and digital PCR method［J］. Food Science,2016,37(16) :235-241.)
［26］金红,孙琪,张斌,等. 利用蛋白质SDS-PAGE电泳方法检测转基因大豆的初步研究［J］.食品研究与开发,2010, 31 (5):148-150.(Jin H, Sun Q, Zhang B, et al. The preliminary study on detecting transgenic soybeans by protein SDS-PAGE method［J］. Food Research and Development,2010, 31 (5):148-150.)
［27］Lipp M, Anklam E, Stare J W, et al. Validation of an immunoassay for detection and quantitation of a genetically modified soybean in food and food fractions using reference materials: Interlaboratory study［J］.Journal of AOAC International,2000(83):919-927.
［28］白卫滨,孙建霞,姜桂传. ELISA方法定量检测转基因大豆及其产品的研究［J］.食品与发酵工业,2007,33 (11) :103-106.(Bai W B, Sun J X, Jiang G C. Study on quantitative detection of genetically modified soybean and processed products by ELISA method［J］. Food and Fermentation Industries,2007,33 (11) :103-106.)
［29］赵晓丽,梁新苗,槐硕,等. 磁性纳米粒子的制备及在转基因大豆检测中的应用［J］.植物检疫,2017,31 (4) :31-33.(Zhao X L, Liang X M, Huai S, et al. Preparation of magnetic nanoparticles for genetically modified soybean detection［J］. Plant Quarantine,2017,31 (4) :31-33.)
［30］李桂华,代红丽,傅黎敏. 高压液相色谱法测定我国大豆种子中维生素E含量［J］.中国粮油学报,2006,21 (3):292-295.(Li G H, Dai H L, Fu L M. The determination of vitamin E content of soybean seeds in China with HPLC［J］. Journal of the Chinese Cereals and Oils Association,2006,21 (3):292-295.)
［31］白月,栾凤侠,高宏伟,等.应用多重 PCR-DHPLC 方法快速检测转基因马铃薯及EH92-527-1 品系鉴定［J］.中国马铃薯,2011,25 (3) :129-134.(Bai Y, Luan F X, Gao H W. Rapid detection of GMO potato and identification of the line EH92-527-1 by multiplex PCR and DHPLC［J］.Chinese Potato Journal,2011,25 (3) :129-134.)
［32］王海龙,杨向东,张初. 近红外高光谱成像技术用于转基因大豆快速无损鉴别研究［J］.光谱学与光谱分析,2016(6):1843-1847.(Wang H L, Yang X D, Zhang C. Fast identification of transgenic soybean varieties based near infrared hyperspectral imaging techonloy［J］. Spectroscopy and Spectral Analysis,2016(6):1843-1847.)
［33］方慧,张昭,王海龙,等. 基于中外光谱技术鉴别转基因大豆的方法研究［J］.光谱学与光谱分析,2017,37 (3) :760-765.(Fang H, Zhang Z, Wang H L, et al. Identification of transgenic soybean varieties using mid-infrared spectroscopy［J］.Spectroscopy and Spectral Analysis, 2017,37 (3) :760-765.)