|Table of Contents|

The Effect of Different Restorative Treatments on the Diversity of Soybean Rhizobia in the Abandoned Land of Ionic Rare Earth Mine(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年01期
Page:
77-83
Research Field:
Publishing date:

Info

Title:
The Effect of Different Restorative Treatments on the Diversity of Soybean Rhizobia in the Abandoned Land of Ionic Rare Earth Mine
Author(s):
LIU Ming-qian1; 2; ZHANG Xin-xin3; MA Jia-hui3; LI Qi-bin3; CHEN Li-jun4; ZHOU Wei2; 3; 5; MA Ling2; 3; 5; CHEN Xiao-yang2; 3; 5
(1.Preparation Office of South China Agricultural Museum, South China Agricultural University, Guangzhou 510642, China; 2.Guangdong Province Research Center of Woody Forage Engineering Technology, South China Agricultural University, Guangzhou 510642, China; 3.College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China; )
Keywords:
Soybean (Glycine max); Ionic rare earth mine; Rhizobia; DGGE; Diversity
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.01.0077
Abstract:
In order to evaluate the effect of different improvement measures on the diversity of rhizobium of soybean (Glycine max) in the abandoned land of ionic rare earth mine, nifH-PCR-DGGE was used to manifestate the diversity of Rhizobia. The results of denaturing gradient gel electrophoresis (DGGE) fingerprint showed that there were differences in the number, the positions and the brightness of bands depended on different restorative treatments, which showed that different soil amendments had a significant effect on the community structure of Rhizobium. The number and diversity index of bands of T4 treatment (fertilizer+biochar+deactivator) were highest, it was best to improve the diversity of rhizobium of soybean. The results of cluster analysis showed that 7 treatments could be divided into four groups, CK was group 1, T1 and T2 were group 2, T4 and T5 were group 3, T3 and T6 were group 4. The combination of biochar and passivator, the combination of bacterial manure and earthworm had the more significant effect on increasing the diversity of rhizobia in root nodules of soybean. According to sequence comparison and phylogenetic tree, soybean is mainly lived in symbiosis with Bradyrhizobium, and also coexisted with Sinorhizobium in the abandoned land of ionic rare earth mine of Meizhou, Guangdong. There was significant positive correlation between the diversity of rhizobium and soil cadmium content, and negative correlation among the diversity of rhizobium and soil organic matter, nitrogen, phosphorus and other nutritional indicators, which suggesting that rhizobium may play an important role in improving the cadmium tolerance of soybean.

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Last Update: 2019-01-22