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Title:: Cellulase Production by Mixed Fungi Solid Fermentation and the Application in Soybean Straw Degradation

Author(s):: SUN Dong-mei; WEN An-yu; LI Xiang; LIN Zhi-wei; XIAO Cui-hong; ZHU Li-wei; （Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region, Heilongjiang Bayi Agricultural University, Daqing 163319, China）

Keywords:: Trichoderma aureoviride; Chaetomium cupreum; Trichoderma viride; Cellulase; Mixed fungi; Soybean Straw; Degradation

PACS:: -

DOI:: 10.11861/j.issn.1000-9841.2019.01.0049

Abstract:: In order to improve the decomposition efficiency of straw returning to field and reduce the diseases aggravation, antagonistic fungi Trichoderma aureoviride, Chaetomium cupreum and cellulose-decomposing fungus T. viride were used to study the cellulase production ability of different strains in mixed culture and its effect on the degradation rate of cellulose and hemicellulose from soybean straw. The results showed that the cellulase activity, cotton enzyme activity and carboxymethyl cellulase activity of three different strains and mixed fermentation at different fermentation time were measured on rice straw and bran as basic medium. The cellulase activity of these three strains mixed fermentaton was the strongest. The cellulase activity was 385.12, 454.30 and 495.12 U, respectively. The changes of cellulose and hemicellulose contents before and after fermentation showed that there were significant differences between the two treatments, and the degradation rates of cellulose and hemicellulose were 66% and 76% respectively. We also found that the degradation rate of soybean straw was the highest when the length of soybean straw was 3 cm and the buried depth was 10 cm by the burying experiment. The degradation rate of soybean straw was increased by more than 50%, the strength of soybean straw was reduced by about 5 times and the puncture force was decreased by adding mixed microbial agent. The results showed that the three strains mixed could be used in the degradation of soybean straw, which could provide effective microbial resources for decomposition of soybean straw.

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Cellulase Production by Mixed Fungi Solid Fermentation and the Application in Soybean Straw Degradation(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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