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Two-dimensional Electrophoresis Pattern Analysis of Leaf Proteins from Soybean under Drought Stress(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2019年01期
Page:
35-40
Research Field:
Publishing date:

Info

Title:
Two-dimensional Electrophoresis Pattern Analysis of Leaf Proteins from Soybean under Drought Stress
Author(s):
ZHEN Wen-xuan YANG Xiao-tong GUO Ge-yang SONG Ying ZHAO Yu-wei ZANG Xin
(School of Life Science, Zhengzhou University, Zhengzhou 450001, China)
Keywords:
Soybean Drought stress Two-dimensional electrophoresis Expression map
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2019.01.0035
Abstract:
Soybean is one of the important crops in China. Proteomics technology is one of the important tools to analyze plant response to stress. To lay the foundation for further identification of homologous proteins of soybean leaf protein in response to drought stress, the leaves of soybean seedlings were used as research objects. The mannitol solution was used to simulate drought stress. After 24 h treatment, the leaf protein powder was extracted for two-dimensional electrophoresis separation and spectral difference analysis. The results showed that the leaf protein was extracted by trichloroacetic acid-acetone precipitation method for two-dimensional electrophoresis analysis to obtain a stable and clear two-dimensional electrophoresis protein map. PD-Quest software was used to analyze the protein expression profiles of soybean leaves under drought stress. A total of 425 soybean leaf protein spots were detected and matched. There were 101 differentially expressed protein spots, among which 60 were up-regulated, and 41 were down-regulated. The results of this study indicated that there were some soybean leaf proteins responded to drought stress.

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Last Update: 2019-01-22