Bioinformatics Analysis of Soybean Argonaute4(PDF)

¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2019Äê01ÆÚ

Page:

25-34

Research Field:

Publishing date:

Info

Title:

Bioinformatics Analysis of Soybean Argonaute4

Author(s):

CAO Le-sheng;  WANG Han-hui;  ZHANG Tian-xu;  XIE Li-nan

£¨School of Life Science, Northeast Forestry Univesity, Harbin 150040, China£©

Keywords:

Soybean;  AGO4;  Bioinformatics;  DNA methylation

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2019.01.0025

Abstract:

In the RNA-directed DNA methylation pathway, the Argonaute4 (AGO4) protein plays a key role in DNA methylation. In order to study Argonaute4 protein in soybean and lay a foundation for the study of methylation in soybean, in this paper, homology analysis of AGO4 gene in Arabidopsis thaliana was performed with NCBI, Phytozome, UniProt KB and other websites, moreover, AGO4 protein and its gene sequence in soybean were confirmed and analyzed. The results indicate that there are three homologous genes of AtAGO4 in soybean, GmAGO4A, GmAGO4B, GmAGO4C respectively, which primary structures, secondary structures and physicochemical property are similar, but the tertiary structures are different, the tertiary structures of GmAGO4C and Arabidopsis AGO4 are more similar. The nucleic acid and protein sequences of the three genes¡¯ exons were analyzed in 302 wild soybean lines, and it was found that the tertiary structures of some soybean lines changed due to mutation.The results of this paper will be helpful for further study on the function of AGO4 in the methylation pathway in soybean.

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