|Table of Contents|

Use Efficiency of Water and Nitrogen of Soybean with Water Stress by Stable Carbon Isotope Discrimination(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年06期
Page:
906-914
Research Field:
Publishing date:

Info

Title:
Use Efficiency of Water and Nitrogen of Soybean with Water Stress by Stable Carbon Isotope Discrimination
Author(s):
(1.College of Water Conservancy and Civil Engineering, Northeast Agricultural University, Harbin 150030, China; 2.Key Laboratory of Effective Utilization of Agricultural Water Resources, Ministry of Agriculture, Harbin 150030, China)
Keywords:
Soybean;  Period of stress;  Leaf water use efficiency(WUE);  Leaf nitrogen use efficiency(NUE);  Arbon isotope discrimination（Δ13C）
PACS:
-
DOI:
1011861/jissn1000-98412018060906
Abstract:
Carbon isotope discrimination (Δ13C) could comprehensively reflect the ratio of intercellular CO2 concentration (Ci) to CO2 concentration (Ca) during a period Ci/Ca reflects the relative amount of photosynthetic rate (Pn) and stomatal conductance (Gs) corresponding to CO2 demand and supply When any of Pn and Gs changes, Ci/Ca will obviously change and ultimately affect water use efficiency(WUE)Therefore, it is possible to infer crop WUE by measuring crop Δ13C Δ13C can also be used to deduce instantaneous water use efficiency (WUEi) and intrinsic water use efficiency (WUEn) at leaf level And it is considered as a reliable method for long-term estimation of crop water use efficiency In addition, leaf nutrient use efficiency (NUE) is the basis of crop growth and high yield, and can be used as a main indicator to reflect crop nutrient absorption characteristics The ratio of soluble sugar content and nitrogen content (C/N) is selected as a crop NUE index in this experiment to reflect the C acquisition and assimilation rate, N distribution and utilization ratio of plant At present, the research on indicator relationship between crop leaf tissue Δ13C and crop WUE and NUE mainly focuse on maize, tomato, and rice under water stress, while there are few studies on soybean with water stress at different growth stages This study combines theoretical analysis with field experiments to analyze the relationship between soybean leaf Δ13C and WUE and NUE at seedling and flowering stage of soybean with water stress, respectively, to reveal the regulation of carbon and nitrogen metabolism in soybean leaves during different growth periods, which can provide theoretical basis for the efficient use of water and nutrient of crop at leaf level The experiment was conducted at the experimental station of Northeast Agricultural University in Harbin, Heilongjiang province in 2017 The leaf Δ13C, photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and the yield were measured, and the values of WUEi, WUEn and WUEET were calculated Results showed that WUE could be improved with moderate water stress during different growth periods of soybean, and WUE and yield were both the highest during flowering period with mild water stress At different stages with water stress, Δ13C in soybean leaves was negatively correlated with WUEi, WUEn and NUE, and the indicative effect of Δ13C on WUEn was superior to WUEi The correlation between leaf Δ13C and WUEi, WUEn and NUE at flowering stage was more significant than that at seedling stage with the same water stress Therefore, the leaf Δ13C at maturity stage of soybean with water stress can be used as an indicator of WUE and NUE of soybean leaves to provide a theoretical basis for improving water use efficiency of soybean by water control

References:

［1］闫永銮, 郝卫平, 梅旭荣,等 拔节期水分胁迫-复水对冬小麦干物质积累和水分利用效率的影响［J］. 中国农业气象, 2011, 32(2):190-195 (Yan Y L, Hao W P, Mei X R, et al., Effects of water stress re-watering at jointing stage on dry matter accumulation and WUE of winter wheat［J］.Chinese Journal of Agronmeteorology,2011,32(2):190-195)
［2］王利彬, 祖伟, 董守坤,等 干旱程度及时期对复水后大豆生长和代谢补偿效应的影响［J］. 农业工程学报, 2015, 31(11):150-156 (Wang L B, Zu W, Dong S K, et al. Effects of drought stresses and times on compensation effect after re-watering in soybean［J］. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(11): 150－156)
［3］褚丽丽 营养生长期水分胁迫和氮素对大豆干物质及产量的影响［J］. 节水灌溉, 2016(7):31-35 (Chu L L Effects of water stress in vegetative growth stages and nitrogen on yield and dry matter of soybean［J］. Water Saving Irrigation,2016(7):31-35.)
［4］陈平，张劲松，孟平，等稳定碳同位素测定水分利用效率——以决明子为例［J］.生态学报，2014，34(19):5453-5459(Chen P, Zhang J S, Meng P, et al. Feasibility analysis on the determination of WUE by stable carbon isotope: Cassia obtusifolia Las an example［J］. Acta Ecologica Sinica,2014, 34(19): 5453-5459.)
［5］Pascual M, Lordan J, Villar J M, et al. Stable carbon and nitrogen isotope ratios as indicators of water status and nitrogen effects on peach trees［J］. Scientia Horticulturae, 2013, 157(3): 99-107
［6］马心灵, 朱启林, 耿川雄，等不同氮水平下作物养分吸收与利用对玉米马铃薯间作产量优势的贡献［J］. 应用生态学报，2017, 28(4):1265-1273(Ma X L, Zhu Q L, Geng C X, et al. Contribution of nutrient uptake and utilization on yield advantage in maize and potato intercropping under different nitrogen application rates［J］. Chinese Journal of Applied Ecology,2017, 28(4): 1265-1273)
［7］Livingston N J, Guy R D, Sun Z J, et al. The effects of nitrogen stress on the stable carbon isotope composition, productivity and water use efficiency of white spruce ［Picea glauca (Moench) Voss］ seedlings［J］. Plant Cell & Environment, 1999, 22(3):281-289
［8］展小云，于贵瑞，盛文萍，等中国东部南北样带森林优势植物叶片的水分利用效率和氮素利用效率［J］. 应用生态学报，2012，23(3):587-594(Zhan X Y, Yu G R, Sheng W P, et al. Foliar water use efficiency and nitrogen use efficiency of dominant plant species in main forests along the North-South Transect of East China［J］.Chinese Journal of Applied Ecology,2012,23(3):587-594)
［9］王利纳，陶洪斌，戴明宏，等包膜尿素对夏玉米产量及碳氮代谢的影响［J］. 玉米科学，2009, 17(2):124-129(Wang L N, Tao H B, Dai M H, et al. Effect of coated urea on grain yield and carbon nitrogen metabolism of summer maize［J］.Journal of Maize Sciences, 2009, 17(2):124-129)
［10］王振昌，郭相平，黄双双，等水稻天然稳定碳、氮同位素组成与产量及品质的关系［J］. 农业机械学报，2016, 47(12):160-171(Wang Z C, Guo X P, Huang S S, et al. Relationships between stable C and N isotope compositions of rice plants, rice yield and quality［J］. Transactions of The Chinese Society of Agricultural Machinery, 2016, 47(12):160-171)
［11］郑利剑，马娟娟，郭飞，等 基于水稳定同位素技术的生物炭对土壤持水性影响分析［J］. 农业机械学报，2016，47(6):193-198(Zheng L J, Ma J, Guo F, et al. Impact of biochar addition on soil water retention based on water stable isotopes［J］. Transactions of The Chinese Society of Agricultural Machinery, 2016, 47(6):193-198)
［12］高暝，黄秦军，丁昌俊，等 美洲黑杨及其杂种F1不同生长势无性系叶片δ13C和氮素利用效率［J］. 林业科学，2013，49(8):51-57(Gao M, Huang Q J, Ding C J, et al. Foliar δ13C and nitrogen use efficient of populus deltoids and the different growth vigor F1 hybrid clones［J］. Forest Research, 2013,49(8):51-57)
［13］鲁如坤土壤农业化学常规分析方法［M］.北京:科学出版社, 1983(Lu R K Methods of soil agricultural chemistry analysis［M］. Beijing:Science Press,1983)
［14］Farouhar G D, Leary M H O, Berry J A On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves［J］.Australian Journal of Plant Physiology, 1982,9(2):121-137
［15］Anderson J E, Kriedemann P E, Austin M P, et al. Eucalypts forming a canopy functional type in dry sclerophyll forests respond differentially to environment［J］. Australian Journal of Botany, 2000, 48(6):759-775
［16］赵宏伟，李秋祝，魏永霞 不同生育时期干旱对大豆主要生理参数及产量的影响［J］. 大豆科学，2006, 25(3):329-332(Zhao H W, Li Q Z, Wei Y X Effect of drought at different growth stages on main physiological parameters and yield in soybean［J］. Soybean Science, 2006, 25(3):329-332)
［17］Naglaa L, Mohamed A E, Ahmed M H, et al. Changes in the water status and osmotic solute contents in response to drought and salicylic acid treatments in four different cultivars of wheat (Triticum aestivum)［J］. Journal of Plant Research, 2012, 125(1):173-184
［18］刘莹，李鹏，沈冰，等 采用稳定碳同位素法分析白羊草在不同干旱胁迫下的水分利用效率［J］. 生态学报，2017, 37(9):3055-3064(Liu Y, Li P, Shen B, et al.．Effects of drought stress on Bothriochloa ischaemum water-use efficiency based on stable carbon isotope［J］．Acta Ecologica Sinica,2017,37(9):3055-3064)
［19］Hu Y, Burucs Z, Tucher S V, et al. Short-term effects of drought and salinity on mineral nutrient distribution along growing leaves of maize seedlings［J］.Environmental & Experimental Botany, 2007, 60(2):268-275
［20］郑恩楠, 杨桦, 陈鹏, 等水氮管理模式下水稻碳氮吸收、土壤呼吸与产量效应［J］.农业机械学报，2018(6):287-295(Zheng E N, Yang H, Chen P, et al. Effects of water and fertilizer management modes on carbon-nitrogen absorption soil respiration and yield of rice［J］. Transactions of The Chinese Society of Agricultural Machinery, 2018(6): 287-295)
［21］赵立琴 干旱胁迫对大豆抗旱生理指标及产量和品质影响［D］.哈尔滨：东北农业大学，2014(Zhao L Q Effect of drought stress on soybean biological drought resistance indexes, yield and quality ［D］.Harbin: Northeast Agricultural University,2014)
［22］薛利红, 杨林章, 范小晖基于碳氮代谢的水稻氮含量及碳氮比光谱估测［J］.作物学报,2006,32(3):430-435 (Xue L H, Yang L Z, Fan X H Estimation of nitrogen content and C/N in rice leaves and plant with canopy reflectance spectra［J］.Acta Agronomica Sinica, 2006,32(3):430-435)
［23］汪可欣，付强，姜辛，等秸秆覆盖模式对玉米生理指标及水分利用效率的影响［J］.农业机械学报,2014,45(12):181-186(Wang K X, Fu Q, Jiang X, et al. Effect of straw mulching mode on maize physiological index and water use efficiency［J］. Transactions of The Chinese Society of Agricultural Machinery, 2014,45(12):181-186)
［24］张忠学, 陈鹏, 郑恩楠,等基于Δ13C分析不同水氮管理对水稻水分利用效率的影响［J］.农业机械学报,2018,49(5):303-312 (Zhang Z X, Chen P, Zheng E N, et al. Effect of different water and nitrogen management on rice leaf water use efficiency based on Δ13C［J］.Transactions of The Chinese Society of Agricultural Machinery, 2018,49(5):303-312)
［25］Iqbal M M, Akhter J, Mohammad W, et al. Effect of tillage and fertilizer levels on wheat yield, nitrogen uptake and their correlation with carbon isotope discrimination under rainfed conditions in north-west Pakistan［J］. Soil & Tillage Research, 2005, 80(1-2):47-57
［26］魏镇华, 杜太生, 张娟, 等利用碳同位素分辨率表征沟灌番茄水分利用效率［J］.农业工程学报,2013,29(7):58-65(Wei Z H, Du T S, Zhang J, et al. Stable carbon isotope discrimination on water use efficiency of field tomato under furrow irrigation［J］. Transactions of the Chinese Society of Agricultural Engineering, 2013,29(7):58-65)
［27］Cabrerabosquet L, Molero G, Bort J, et al. The combined effect of constant water deficit and nitrogen supply on WUE, NUE and Δ13C in durum wheat potted plants［J］. Annals of Applied Biology, 2007, 151(3):277-289
［28］Farquhar G D, And J R E, Hubick K T Carbon isotope discrimination and photosynthesis［J］. Annual Review of Plant Physiology and Plant Molecular Biology, 1989, 40(40):503-537
［29］Araus J L, Villegas D, Aparicio N, et al. Environmental factors determining carbon isotope discrimination and yield in durum wheat under mediterranean conditions［J］. Crop Science, 2003, 43(1):170-180

Memo

Memo:
-
Last Update: 2018-12-04