Effect of nodVW Genes on Symbiotic Nitrogen Fixation of Sinorhizobium fredii HH103(PDF)

¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2018Äê06ÆÚ

Page:

871-875

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Publishing date:

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Title:

Effect of nodVW Genes on Symbiotic Nitrogen Fixation of Sinorhizobium fredii HH103

Author(s):

AN Qi1; 2;  WU Hao-qiong1; 2;  YIN Bo1; 2;  YUAN Tao1; 2;  YUAN Ming3;  CAO Ya-bin1; 2;  NIU Yan-bo1; 2;  MA Yin-peng1

£¨1.Institute of Microbiology£¬Heilongjiang Academy of Sciences£¬Harbin 150010£¬China£» 2.Institute of Advanced Technology£¬Heilongjiang Academy of Sciences£¬Harbin 150020£¬China£» 3.Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences£¬Qiqihar 161006£¬China£©

Keywords:

Two component regulatory system;  nodVW;  Recombinant rhizobium;  Nodulation and nitrogen fixation

PACS:

-

DOI:

1011861/jissn1000-98412018060871

Abstract:

In this study, Bradyrhizobium japonicum USDA110 was used as the starting strain The two component regulatory genes nodVW from Bradyrhizobium japonicum USDA110 were amplified and introduced to Sinorhizobium fredii HH103 and HH103 nodD1 mutant strains in order to study the effect of nodVW on symbiotic nitrogen fixation of the recombinant strains to soybean genotype NF-16 The results showed that the introduction of the two-component control system NodVW from Bradyrhizobium japonicum USDA110 exert negative effect on soybean plants, which significantly reduced the height and fresh weight of soybean plants In the meanwhile, the number of nodules at soybean roots decreased significantly The results indicated that the introduction of NodVW hindered the pathway and inhibits the nodulation and nitrogen fixation of Sinorhizobium fredii HH103 accordingly

References:

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