|Table of Contents|

Bioinformatics Analysis of Vegetable Soybean Sucrose Transporter
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《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年06期
Page:
866-870
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis of Vegetable Soybean Sucrose Transporter
Author(s):
ZHANG Yu-mei HU Run-fang LIN Guo-qiang
(Crop Sciences Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China)
Keywords:
Vegetable soybeanSUTBioinformatics analysis GmSUT
PACS:
-
DOI:
1011861/jissn1000-98412018060866
Abstract:
In order to reveal the construction features of vegetable soybean sucrose transporter in the molecular level and provide a theoretical basis for increasing the activity of its expression.Soybean SUT gene whose sequence is 1 634 bp in length was cloned by RT-PCR and the sequence in coding region of SUT is 1 566 bp,a polypeptide was encoded by 521 amino acid residues.Bioinformatics analysis showed that the theory value of pI and Mw was 933 and 5519 kD, respectively SUT was a hydrophobic protein The secondary structure of SUT various amino acid residues were predicted by SOMPA, which was as follows alpha helix accounting for 4511%, extended strand for 1420%, random coil for 3647% and β-turn for 422% According to the amino acid sequence and structural analysis,it showed that SUT protein contained one conserved domain (MFS_2 domain) and twelve transmembrane regions This protein was no signal peptide analysised by SignalP Phylogenetic tree analysis showed that the GmSUT had the closest genetic relationship with Vignaradiata var radiata and the homology was 9328% The alignment between this protein and 9 SUC proteins of Arabidopsis thaliana showed that GmSUT was closely related to AtSUC2 and belonged to the SUT1 subfamily

References:

[1]Barker L,Kühn C, Weise A, et al. SUT2, a putative sucrose sensor in sieve element [J]. Plant Cell, 2000(12):1153-1164
[2]Williams L E, Lemoine R, Sauer N Sugar transporters inhigher plants-a diversity of roles and complex regulation [J].Trends in Plant Science, 2000, 5(7):283-290
[3]Hirose T, Zhang Z, Miyao A, et al. Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected[J] Journal of Experimental Botany, 2010, 61(13):3639-3646
[4]Kühn C, Grof C P L Sucrose transporters of higher plants [J]. Current Opinion in Plant Biology, 2010, 13(3):288-298
[5]Aoki N, Hirose T, Scofield G N, et al. The sucrose transporter gene family in rice [J]. Plant and Cell Physiology, 2003, 44(3): 223-232
[6]Ibraheem O, Dealtry G, Roux S, et al. The effect of drought and salinity on the expressional levels of sucrose transporters in rice (Oryza sativa Nipponbare) cultivar plants [J]. Plant Omics Journal, 2011, 4(2):68-74
[7]Mimura M, Coyne C J, Bambuck M W, et al. SSR diversity of vegetablesoybean [Glycine max (L) Merr] [J]. Genetic Resources and Crop Evolution, 2007, 54:497-508
[8]Czaikoski K, Leite R S, Mandarino J M G, et al. Canning of vegetable-type soybean in acidified brine: Effect of the addition of sucrose and pasteurisation time on color and other characteristics [J]. Industrial Crops and Products, 2013, 45:472-476
[9]Kim S Y, Kim B M, Kim J B, et al. Effect of steaming, blanching, and high temperature/ high pressure processing on the amino acid contents of commonly consumed Korean vegetables and pulses[J]. Prevention Nutrition and Food Science,2014, 19:220-226
[10]徐有,王凤敏,默邵景,等我国菜用大豆的研究现状与发展趋势[J].河北农业科学,2012,16(4):42-45(Xu Y, Wang F M, Mo S J, et al. Research status and development tendency of vegetable soybean in China [J]. Journal of Hebei Agricultural Sciences, 2012,l6(4):42-45)
[11]张彩英,常文锁,李喜焕,等种植密度和施肥对莱用大豆产量性状的效应研究[J].中国农学通报,2005,21(7):190-192(Zhang C Y, Chang W S, Li X H, et al. Effect of plant density and phosphorus application rate in yield of vegetable soybean [J]. Chinese Agricultural Science Bulletin, 2005,21(7):190-192)
[12]张玉梅, 赵晋铭, 王明军, 等 南方菜用大豆资源营养品质性状的遗传变异[J]. 大豆科学,2006, 25(3):239-243 (Zhang Y M, Zhao J M, Wang M J, et al. Genetic variance of nutritional quality of vegetbale soybean germplasm of Glycine max Merr in southern China [J]. Soybean Science, 2006, 25(3): 239-243)
[13]Li Y S, Ming D, Zhang Q Y , et al. Greater differences exist in seed protein, oil, total soluble sugar and sucrose content of vegetable soybean genotypes [Glycine max (L) Merrill] in Northeast China [J]. Australian Journal of Crop Science, 2012, 6(12):1681-1686
[14]Petersen T N, Brunak S, Heijne V,et al.SignalP 40: Discriminating signal peptides from transmembrane regions[J]. Nature Methods, 2011,8(10):785-786
[15]Sauer N, Ludwig A, Knoblauch A, et al. AtSUC8 and AtSUC9 encode functional sucrose transporters but the closely related AtSUC6 and AtSUC7 genes encode aberrant proteins in different Arabidopsis ecotypes[J].The Plant Journal, 2004, 40(1):120-130
[16]Vaughn M W, Harrington G N, Bush D R Sucrose-mediated transcriptional regulation of sucrose symporter activity in the phloem [J].Proceedings of the National Academy of Science of the United States of America, 2002, 99(16):10876-10880
[17]Srivastava A C, Ganesan S, Ismail I O, et al. Functional characterization of the Arabidopsis AtSUC2 sucrose/H+ symporter by tissue specific complementation reveals an essential role in phloem loading but not in long distance transport [J].Plant Physiology, 2008, 148(1):200-211
[18]Riesmeier J W, Willmitzer L, Frommer W B Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning [J]. Embo Journal, 1994, 13(1):1-7
[19]Kühn C, Quick W P, Schulz A, et al. Companion cell-specific inhibition of the potato sucrose transporter SUT1 [J]. Plant Cell Environment, 1996, 19(10):1115-1123
[20]Burkle L, Hibberd J M, Quick W P, et al. The H+-sucrose cotransporter NtSUT1 is essential for sugar export from tobacco leaves [J]. Plant Physiology, 1998, 118(1):59-68
[21]张玉梅, 胡润芳, 陈宇华, 等 菜用大豆籽粒代谢物的相关性分析[J]. 大豆科学, 2018, 37(2):231-238 (Zhang Y M, Hu R F, Chen Y H, et al. Correlation analysis between metabolites in vegetable soybean seeds [J]. Soybean Science, 2018, 37(2):231-238 )
[22]张秋英, 李彦生, 刘长锴, 等 菜用大豆食用品质关键组分及其积累动态研究[J]. 作物学报,2015, 41(11):1692-1700 (Zhang Q Y, Li Y S, Liu C K, et al. Key components of eating quality and their dynamic accumulation in vegetable soybean varieties [Glycine max (L) Merr] [J]. Acta Agronomica Sinica,2015, 41(11): 1692-1700)
[23]Zhang Y M, Hu R F, Li H W, et al. Proteomics changes in filling seeds of vegetable soybean[J]. Hort Science, 2016, 51(11):1397-1401

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Last Update: 2018-12-04