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Method Optimization for the Determination of Lipoxygenase in Soybean Milk and the Evaluation of Its Kinetics(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年05期
Page:
769-775
Research Field:
Publishing date:

Info

Title:
Method Optimization for the Determination of Lipoxygenase in Soybean Milk and the Evaluation of Its Kinetics
Author(s):
XIA Ning1; 2 ;  WANG Xiao-qi2;  YAN Wen-bing2;  ZHANG Zhi-hua3;  GUO Shuang2;  YANG Wen-jun2;  ZHAO Xue2;  SHI Yan-guo1
(1.School of Food Engineering, Harbin University of Commerce, Harbin 150076, China; 2.Key Laboratory of Soybean Biology, Northeast Agricultural University, Harbin 150030, China; 3.School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)
Keywords:
soybean milk;  LOX;  Enzymatic reaction kinetics;  Inhibitors;  Activator
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2018.05.0769
Abstract:
Based on the method of determining the activity of LOX enzyme in soybean, we optimized the stability of the method of determining the activity of LOX in raw soybean milk, and studied the effect of heat treatment and different concentration of soybean milk on the activity of LOX in this paper. The maximum reaction rate of LOX, the Michlet rate constant and the kinetic parameters of enzyme reaction such as different metal ion inhibitors and activators were determined. The results showed that the change trend of LOX activity was obvious when the sample was diluted 300 times. The results were stable, and the results of soybean pulping in 10 different regions were determined at 300 times dilution times by different heating temperatures. The activity of LOX in soybean milk was highest at 40℃ and completely inactivated when heated to 80℃. Compared with other reported methods, the optimized method can measure the activity of LOX in soybean milk of different soybean varieties quickly and accurately, and the experimental results are reproducible and stable. By studying the kinetics of enzymatic reaction of LOX, the maximum reaction rate of LOX was determined: Vmax=1.99ΔA·min-1,R2=0.942 8,Km=452.49 μmol·L-1. In metal ions, Ca2+ and Mg2+ had an inhibitory effect on LOX. At the concentration of 0.002 mol·L-1, the LOX was inhibited, and when the concentration of zinc ion was greater than 0.004 mol·L-1, the LOX was activated.

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Last Update: 2018-10-08