|Table of Contents|

Effect of Bacillus Subtilis 8-32 on Soil Enzymes and Microflora of Potted Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年05期
Page:
762-768
Research Field:
Publishing date:

Info

Title:
Effect of Bacillus Subtilis 8-32 on Soil Enzymes and Microflora of Potted Soybean
Author(s):
YANG Ke-xin WANG Huan LIU Xue-jiao SHI Xiang-zhe JIA Tian-hui GAO Tong-guo
(College of Life Science, Agricultural University of Hebei, Baoding 071001, China)
Keywords:
Soybean root rot Biocontrol Bacillus subtilis Soil enzymes Microflora
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2018.05.0762
Abstract:
In previous study, a strain Bacillus subtilis 8-32, which had strong antagonistic ability against to Fusarium oxysporum was screened, its biocontrol effect reached to 32.08%. In this experiment, further study was carried out by pot experiments. We studied the changing of soil enzyme system and microflora after inoculated different concentration of Bacillus subtilis 8-32. The results showed that: Bacillus subtilis 8-32 increased the activity of soil urease by 16.9%-66.7%, protease activity increased by 88.8%-131.0%, acid phosphatase activity increased by 20.6%-51.8%, alkaline phosphatase activity increased by 7.5%-14.6% and bacteria in soil increased by 8.5%-72.5%, the quantity of soil fungi inhibition rate reached to 37.2%, the number of actinomyces increased obviously. It indicated that strain 8-32 has significant control effect on soybean root rot caused by Fusarium oxysporum, and has potential for further application.

References:

[1]姜伊. 大豆根腐病主要致病菌种类及防控技术[D]. 哈尔滨: 黑龙江大学, 2014. (Jiang Y. Main pathogenic bacteria and control techniques of soybean root rot[D]. Harbin: Heilongjiang University, 2014.)
[2]马淑梅.黑龙江省大豆根腐病致病病原种类分布及抗病种质鉴定[J].中国农学通报,2012,28(27):230-235. (Ma S M. Pathogenic pathogen categories distribution and germplasm resistance identification of soybean root rot in Heilongjiang province[J]. Chinese Agricultural Science Bulletin, 2012,28(27):230-235.)
[3]成瑢,董铮,李魏,等.大豆根腐病研究进展[J].中国农学通报,2016,32(8):58-62. (Cheng R, Dong Z, Li W, et al. Research progress of soybean root rot [J]. Chinese Agricultural Science Bulletin, 2016,32(8):58-62.)
[4]张丽,耿肖兵,王春玲,等.黑龙江省大豆镰孢根腐病菌鉴定及致病力分析[J].植物保护,2014,40(3):165-168. (Zhang L, Geng X B, Wang C L, et al. Identification and virulence of Fusarium spp. causing soybean root rot in Heilongjiang province[J]. Plant Protection, 2014,40(3):165-168.)
[5]王晓艳,文景芝.东北三省大豆根腐镰孢菌种类及其致病力分析[J].中国油料作物学报,2011,33(4):391-395. (Wang X Y, Wen J Z. Species and pathogenicity of Fusarium causing soybean root rot in northeast China[J]. Chinese Journal of Oil Crop Sciences, 2011,33(4):391-395.)
[6]王丹秋,李焕玲,王惟萍,等.李宝聚博士诊病手记(五十七)枯草芽孢杆菌菌剂防治蔬菜病害应用技术研究[J].中国蔬菜,2013(5):23-26. (Wang D Q, Li H L, Wang W P, et al. Dr. Li Baoju′s record of diagnosis and treatment (fifty-seven) application of Bacillus subtilis inoculant to control vegetable diseases[J]. China Vegetables, 2013(5):23-26.)
[7]杨洁. 微生物制剂对西瓜枯萎病的防治研究[D].郑州: 郑州大学,2017. (Yang J. Study on the prevention and treatment of watermelon Fusarium wilt by microorganism preparation[D]. Zhengzhou: Zhengzhou University, 2017.)
[8]杨赞. Paenibacillus polymyxa SIIA-1408产抗菌活性物质的研究[D]. 成都: 成都学院,2017. (Yang Z. Study on antibacterial substances produced by Paenibacillus polymyxa SIIA-1408[D]. Chengdu: Chengdu University, 2017.)
[9]李晓舟.枯草芽孢杆菌B504菌株形态学及生理生化性状研究[J].农民致富之友, 2015(20):75-76. (Li X Z. Morphological, physiological and biochemical characteristics of Bacillus subtilis strain B504[J]. Friends of the Farmers, 2015(20):75-76.)
[10]刘培福. 枯草芽孢杆菌D221发酵条件优化及对辣椒根腐病防治效果初探[D].哈尔滨: 东北农业大学,2011. (Liu P F. Studies on optimization of fermentation conditions and biocontrol effects against Fusarium solan of Bacillus subtilis D221[J].Harbin: Northeast Agricult ural University, 2011.)
[11]李晶,杨谦.生防枯草芽孢杆菌的研究进展[J].安徽农业科学,2008(1):106-111,132. (Li J, Yang Q. Research progress on biocontrol Bacillus subtilis[J].Journal of Anhui Agricultural Sciences, 2008(1):106-111,132.)
[12]王佳. 大豆根腐病生防菌的鉴定及发酵条件的优化[D]. 哈尔滨: 黑龙江大学, 2010. (Wang J. Identification of biocontrol bacteria against soybean root rot and optimization of fermentation conditions[D]. Harbin: Heilongjiang University, 2012.)
[13]高同国,李术娜,张冬冬,等.大豆根腐病生防细菌优势菌株的筛选、鉴定及生防效果验证[J].大豆科学,2015,34(4):661-665. (Gao T G, Li S N, Zhang D D, et al. Screening and identification of biocontrol dominant bacterium against to soybean root rot[J]. Soybean Science, 2015,34(4):661-665.)
[14]胡云云,高同国,张冬冬,等.大豆根腐病拮抗菌枯草芽孢杆菌8-32抗菌物质性质的初步研究[J].湖北农业科学,2016,55(4):917-920. (Hu Y Y, Gao T G, Zhang D D, et al. A preliminary study on physicochemical properties of antibacterial substances from Bacillus subtilis 8-32 against soybean root rot[J]. Hubei Agricultural Sciences, 2016,55(4):917-920.)
[15]高同国,张冬冬,郭晓军,等.芽孢杆菌8-32对大豆根腐病的生防效果[J].湖北农业科学,2015,54(18):4489-4492. (Gao T G, Zhang D D, Guo X J, et al. Biocontrol effect of Bacillus sp. 8-32 against soybean root rot[J]. Hubei Agricultural Sciences, 2015,54(18):4489-4492.)
[16]郭振,王小利,徐虎,等.长期施用有机肥增加黄壤稻田土壤微生物量碳氮[J].植物营养与肥料学报,2017,23(5):1168-1174. (Guo Z, Wang X L, Xu H, et al. A large number of long-term application of organic fertilizer can effectively increase microbial biomass carbon and nitrogen in yellow paddy soil[J]. Journal of Plant Nutrition and Fertilizer, 2017,23(5):1168-1174.)
[17]马媛媛,李玉龙,来航线,等.连作番茄根区病土对番茄生长及土壤线虫与微生物的影响[J].中国生态农业学报,2017, 25(5):730-739. (Ma Y Y, Li Y L, Lai H X, et al. Effect of sick rhizosphere soil under tomato continuous cropping on soil nematodes, microbes and tomato growth[J]. Chinese Joural of Eco-Agriculture, 2017, 25(5):730-739.)
[18]王春燕,何念鹏,吕瑜良.中国东部森林土壤有机碳组分的纬度格局及其影响因子[J].生态学报,2016,36(11):3176-3188. (Wang C Y, He N P, Lyu Y L. Latitudinal patterns and factors affecting different soil organic carbon fractions in the eastern forests of China[J]. Acta Ecologica Since, 2016,36(11):3176-3188.)
[19]张晓燕. 内蒙古乌海地区土壤放线菌的分离及对马铃薯3种病原真菌的拮抗研究[D]. 呼和浩特: 内蒙古农业大学,2016. (Zhang X Y. Isolation of soil actinomycetes from Wuhai area of Inner Mongolia and their antagonistic effects on 3 pathogenic fungi of potato[D]. Hohhot: Agricultural University Of the Inner Mongol, 2016.)
[20]詹庆,曹雅丽.土壤中放线菌的分离与应用[J].现代园艺,2017(3):24-25. (Zhan L, Cao Y L. Isolation and application of actinomycetes in soil[J]. Modern Horticulture, 2017(3):24-25.)
[21]范文艳,陈瑾,姜述君,等.复合生防菌群对连作大豆根际土壤可培养微生物区系的影响[J].中国油料作物学报,2012,34(3):286-293. (Fang W Y, Chen J, Jiang S J, et al. Effect of combined biocontral agents on soybean rhizosphere culturable microbial flora from continuous soybean cropping soil[J]. Chinese Journal of Oil Crop Sciences, 2012,34(3):286-293.)
[22]陈香,唐彤彤,孙星,等.对黄瓜枯萎病具防效的海洋源芽孢杆菌Y3F的鉴定[J].微生物学通报,2017,44(10):2370-2379. (Chen X, Tang T T, Sun X, et al. Identification of marine bacillus isolate Y3F suppressing fusarium wilt of cucumber[J]. Microbiology China, 2017,44(10):2370-2379.)

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Last Update: 2018-10-08