|Table of Contents|

Cloning and Expression Analysis of Soybean GmSAMDC1-encoding Gene(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年05期
Page:
672-680
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Soybean GmSAMDC1-encoding Gene
Author(s):
REN Rui1; 2;  YANG Feng-xi2;  WANG Tao1; 3;  GAO Le1;  ZHI Hai-jian1;  LI Kai1
(1.Soybean Research Institute of Nanjing Agricultural University/Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture /National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 2.Environmental Horticulture Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 3. Institute of Cereal and Oil Crops, Handan Academy of Agricultural Sciences, Handan 056001, China)
Keywords:
Soybean;  GmSAMDC1;  Tolerance to stress;  Cis-acting elements;  Subcellular localization
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2018.05.0672
Abstract:
S-adenosine methionine decarboxylase(SAMDC), the key enzyme of spermine biosynthesis, is one of the speed-limit enzymes in the biosynthesis of polyamine playing an important regulatory role in plant tolerance to stress. To explore the structure and expression characteristics of soybean SAMDC-encoding genes, the gene GmSAMDC1（Glyma.02G128000） located on chromosome 2 was cloned from the resistance soybean cultivar Kefeng 1 in this study. Results showed that the gene GmSAMDC1, of which complete ORF length was 1 068 bp, and encoded a protein of 355 amino acids containing a ‘SAM_decarbox’ domain. For this protein, its isoelectric point (pI) was 4.86, and the relative molecular mass was 38 987.13 Da, containing no transmembrane region as the hydrophilic protein. There were 8 GmSAMDC1homologous in soybean, and the genetic relationship of the SAMDC protein-coding genes in red clover (PNY09439.1, 82.64%) and Arabidopsis thaliana (AtSAMDC3, 67.22%) was closest. The GmSAMDC1promoter sequence contains many cis-acting elements such as defense and stress response elements, plant hormone response elements, and light response components. The expression of GmSAMDC1in flowers was the highest, which was similar to the tissue specific expression pattern of Glyma.01G071300(sequence similarity is 94.6%), Glyma.18G278800 (66.8%) and Glyma.08G25580 (66.5%). Fusion protein Glyma.02G128000-GFP was expressed in cell membrane and cytoplasm. The results of this study lay a theoretical basis for further elucidation of the role of soybean GmSAMDC1in the process of soybean tolerance to stresses.

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Last Update: 2018-10-08