Cloning and Expression Analysis of Transcription Factors GmWRKY4 in Soybean(PDF)

¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2018Äê04ÆÚ

Page:

539-544

Research Field:

Publishing date:

Info

Title:

Cloning and Expression Analysis of Transcription Factors GmWRKY4 in Soybean

Author(s):

WANG Zhao-yu¹; ZHEN Zhen¹; LI Ya-lin¹; WANG Cui-xia²; LIU Si-min¹; GUO Yun-tong¹; YAN Rui-xian¹; LIU Jian-feng¹

(1.College of Life Science, Hebei University,Baoding 071002,China; 2.Agriculture and Animal Husbandry Bureau of Jizhou District,Hengshui 053200,China)

Keywords:

Glycine max; GmWRKY4; Bioinformatics;  Abiotic stres; Hormone induction

PACS:

-

DOI:

-

Abstract:

In order to understand how WRKY gene family participates in soybean defensive responses and regulating the growth and development of soybean, Tiefeng 29 was used as the research material to obtain the open reading frame (ORF)1 083 bp of soybean gene GmWRKY4, which encoded 360 amino acid polypeptide. Further bioinformatics analysis revealed that the amino acid sequence of GmWRKY4 had two structural conserved domains (WRKY) and C2H2 type zinc finger structure, so it belonged to the class I of gene family. The homology of amino acid sequence between GmWRKY4 and GsWRKY was 99%. The phylogenetic tree analysis showed that the GmWRKY4, Arabidopsis AtWRKY3 and AtWRKY4 belonged to the same class. It was also found that the GmWRKY4 was involved in responses to different stress, including NaCl, PEG, low-tempture and ACC,SA,JA induction. This study would lay a foundation for the family structure and functional research of soybean WRKY transcription factors, and further provide scientific basis for research of soybean resistance and molecular breeding.

References:

Memo

Memo:

-

Common functions