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Optimization for the Transformation System of Soybean Cotyledon Node(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年04期
Page:
531-538
Research Field:
Publishing date:

Info

Title:
Optimization for the Transformation System of Soybean Cotyledon Node
Author(s):
LI Dong-mei;  CHEN Wei;  LI Yong-guang;  LI Wen-bin
（Soybean Reasearch Institute Key Laboratory of Soybean Biology in Chinese Ministry of Education/Agricultural Ministry’s Northeast Areal Key Laboratory of Soybean Biology and Genetic Breeding，Northeast Agricultural University, Harbin 150030, China）
Keywords:
Agrobacterium-mediated;  Soybean cotyledon section;  Ultrasonic;  Vacuum
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2018.04.0531
Abstract:
For improving the efficiency and establishing a stable system, the process of soybean cotyledonary transformation was optimized in this study. Cluster buds of cotyledons with four different genotypes were chosen for analyzing the transformation efficiency by b-glucuronidase (GUS) staining. The results indicated that treatments with ultrasonic and vacuum were positive regulators for Agrobacterium infection. GUS staining showed that, the genetic transformation efficiencies of four genotypes were improved after treatment for different periods of time. The preferred conditions for both treatments were ultrasonic for 30 s and vacuum for 2 min, and conversion efficiency increased by 8%~42% and 16%~41%, respectively, which applied for all of the above four genotypes. At the earlier stage of induction of clustered buds, the optimal screening concentration of Phosphinothricin (PPT) was 0.2 mg·L-1, and the conversion efficiency increased by 18%~33%.

References:

［1］于洋, 侯文胜, 韩天富. 农杆菌介导大豆遗传转化技术的研究进展［J］. 大豆科学, 2010, 29(4): 696-701. (Yu Y, Hou W S,Han T F. Research progress on agrobacterium mediated genetic transformation of soybean［J］. Soybean Science,2010, 29(4):696-701.)
［2］任海洋, 南海洋, 曹东, 等.大豆转基因技术研究进展［J］.东北农业大学学报, 2012,43(7):6-12.(Ren H Y,Nan H Y,Cao D,et al. Research progress in soybean transgenic technology［J］. Journal of Northeast Agricultural University, 2012,43(7): 6-12.)
［3］Byrnc M C, McDonnell R E, Wright M S, et al. Strain and cultivar specificity in the Agrobacterium-soybean interaction［J］. Plant Cell, Tissue and Organ Culture, 1987, 8: 3-15.
［4］de Block M, Herrera-Estrella L, van Montagu M, et al. Expression of foreign genes in regenerated plants and their progeny［J］. EMBO Journal, 1984, 3:1681-1690.
［5］Horsch R, Bfraley R, Trogers S G, et al. Inheritance of functional foreign genes in plants［J］. Science,1984,233:496-498.
［6］Hinchee M A W, ConnorWard D V, Newell C A, et al. Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer［J］. Nature Biotechnology, 1988, 6: 915-922.
［7］McCabe D E, Swain W F, Martinell B J, et al. Stable transformation of soybean(Glycine max) by particle acceleration［J］. Nature Biotechnology, 1988, 6:923-926.
［8］Amoah B K, Wu H, Sparks C, et al. Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue［J］. Journal of Experimental Botany, 2001, 52(358): 1135-1143.
［9］Joersbo M, Brunstedt J. Sonication : A new method for gene transfer to plants［J］. Physiologia Plantarum, 1992, 85: 230-234.?
?［10］Trick H N, Finer J J. Sonication- assisted Agrobacterium mediated transformation of soybean ［Glycine max (L.) Merrill］ embryogenic suspension culture tissue［J］. Plant Cell Report, 1998, 17(6): 482- 488.
［11］Cheng T Y, Saka T, Voqui-Dinh T H. Plant regeneration from soybean cotyledonary node segments in culture［J］. Plant Sicience Letter, 1980, 19:91-99.
［12］Meurer C A, Dinkins R D, Collins G B. Factors affecting soybean cotyledonary node transformation［J］. Plant Cell Reports, 1998, 18: 180-186.
［13］Donaldson P A, Simmonds D H. Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean［J］. Plant Cell Reports, 2000, 19:478-484.
［14］Cho H J, Widholm J M. Agrobacterium tumefaciens-mediated transformation of the legume Astragalus sinicus using kanamycin resistance selection and green fluorescent protein expression［J］. Plant Cell, Tissue and Organ Culture, 2002, 69: 251-258.
［15］Paz M, Shou H X, Guo Z B, et al. Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant［J］. Euphytica, 2004, 136: 167-179.
［16］Chen S Y. High-efficiency Agrobacterium-mediated transformation of soybean［J］. Acta Botanica Sinica, 2004, 46 (5): 610-617.
［17］Zhang Z, Xing A, Staswick P, et al. The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean［J］. Plant Cell, Tissue and Organ Culture, 1999, 56: 37-46.
［18］Zeng P, Vadnais D A, Zhang Z, et al. Refined glufosinate selection in Agrobacterium-mediated transformation of soybean ［Glycine max(L.) Merrill］［J］. Plant Cell Report, 2004 22: 478-482.
［19］Trick H, Finer J. Sonication-assisted Agrobacterium-mediated transformation of soybean ［Glycine max (L.) Merrill］ embryogenic suspension culture tissue［J］. Plant Cell Reports, 1998, 17: 482-488.?
［20］Paul C, Dennis E M C, William F S. Stable transformation of soybean callus by DNA-coated gold particles［J］. Plant Physiol,1988,87(3):671-674.
［21］Olhoft P, Flagel L, Donovan C, et al. Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method［J］. Planta, 2003, 216(5): 723-735.?
［22］Olhoft P O, Lin K L, Galbraith J G, et al. Therole of thiol compounds in increasing Agrobacterium-mediated transformation of soybean cotyledonary-node cells［J］. Plant Cell Reports, 2001, 20(8): 731-737.
［23］Zupan J R, Zambryski P. Transfer of T-DNA from Agrobacterium to the plant cell［J］. Plant physiology, 1995,107: 1041-1047.

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Last Update: 2018-07-31