Overexpression of PpCuZnSOD Gene Improves Salt Tolerance in Transgenic Soybean(PDF)

¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2018Äê04ÆÚ

Page:

525-530

Research Field:

Publishing date:

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Title:

Overexpression of PpCuZnSOD Gene Improves Salt Tolerance in Transgenic Soybean

Author(s):

YANG Yan-li;  LI Da-hong; LI Hong-yan

(School of Biotechnology and Food Engineering, Huanghuai University, Zhumadian 463000, China)

Keywords:

Overexpression;  PpCuZnSOD;  Salt tolerance;  Soybean

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2018.04.0525

Abstract:

In order to study the enhanced effect of CuZnSOD encoding gene of peach on salt tolerance of soybean, a full-length cDNA of a CuZnSOD from peach(Prunus persica L.) PpCuZnSOD was cloned, and the target gene is introduced into the soybean genome via Agrobacterium tumefaciens. The transgenic plants was detected by PCR, Southern blot and quantitative real time PCR. Two transgenic soybean lines of Tª­2 generation were recruited and treated with 150 mmol¡¤L-1 NaCl, which simulating salt conditions. The results showed that transgenic soybean plants had high salt tolerance. The seed germination rates, survival rate and the contents of chlorophyll were higher than that of wild-type. Transgenic plants had more high SOD, POD and CAT activities, while less MDA content under salt stress compared with wild-type plants. Our results substantiate that increased levels of SOD activity brought about by overexpression of PpCuZnSOD gene may play an important role in ameliorating oxidative injury induced by salt stress.

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