|Table of Contents|

Cloning and Sequence Analysis of Trypsin Inhibitor Gene from Wild Soybean (Glycine soja Sieb.et Zucc) in Jimo(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年04期
Page:
517-524
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of Trypsin Inhibitor Gene from Wild Soybean (Glycine soja Sieb.et Zucc) in Jimo
Author(s):
XU Yi-rong;  ZHU Yan;  ZHANG Yan-ru;  WANG Min;  ZHANG Li
(College of Food Sciences, Ocean University of China, Qingdao 266003, China)
Keywords:
Glycine soja Sieb.et Zucc;  Trypsin inhibitor;  Gene;  Bioinformatics
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2018.04.0517
Abstract:
In order to analyze the specificity of wild soybean trypsin inhibitor in order to analyze the the molecular level, the full-length sequence of Kunit type trypsin inhibitor (KTI) including 654 bp and Bowman-Birk trypsin inhibitor (BBI) including 357 bp were amplified from Jimo wild soybean (Glycine soja Sieb.et Zucc) by RT-PCR technology, and bioinformatics analysis was performed on the two genes. The gene were sequenced and identified by GenBank Accession No. AB112031.1 and GenBank Accession No. AB081833.1. Homology analysis based on Jimo wild soybean KTI gene and BBI gene showed that both genes belonged to serine protease inhibitor gene family. The results showed that the similarity of KTI gene was 99% among Jimo wild soybean, Glycine soja (No. AB308134.1) and Glycine max (No. EF541136.1)，including six conserved motifs. The similarity of BBI gene was 99%, among Jimo wild soybean, Glycine soja (No. AB081834.1) and Glycine max (NM_001250058.3), including five conserved motifs. The secondary and three-dimensional structure analysis showed significant differences from cultivated soybean.

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Last Update: 2018-07-31