|Table of Contents|

Metabolic Response of the Soybean Pod Borer (Leguminirora glycinioorella) During Diapause to Temperature(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年03期
Page:
415-423
Research Field:
Publishing date:

Info

Title:
Metabolic Response of the Soybean Pod Borer (Leguminirora glycinioorella) During Diapause to Temperature
Author(s):
LI Dong-po;  HAN Lan-lan;  ZHANG Xu-xia;  ZHAO Kui-jun;  ZHANG Wen-lin;  GAO Yun-lei;  DENG Shi-qun;  ZHANG Ao-nan
(College of Agriculture, Northeast Agricultural University, Harbin 150030, China)
Keywords:
Diapause;  Soybean pod borer;  Induction temperatures;  Metabolic enzymes;  Enzyme activity
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2018.03.0415
Abstract:
The purpose was to clarify the metabolic changes during the period of nondiapause-diapause-diapause elimination of soybean pod borer (Leguminirora glycinioorella), measure the occurrence period of soybean pod borer accurately, grasp the timing of prevention and control, and then achieve the purpose of reducing the use of pesticides. Trehalase, sorbitol dehydrogenase, pyruvate kinase and peroxidase are main metabolic enzymes related to antifreeze and diapause of soybean pod borer. This study investigated the changes of those enzyme activity in soybean pod borer before and after diapause, treated with different diapause-inducing temperatures and different stages of diapause period at different temperatures(4, 13, 18, 23, 25℃ and outdoor). The results showed that the activity of the four metabolic enzymes of soybean pod borer diapaused under natural conditions were all significantly different compared with the pre-diapause activity of soybean pod borer. Among them, the activity of trehalase, sorbitol dehydrogenase, pyruvate kinase decreased by 0.99, 2.85 and 7.86 U·mg-1, respectively, the peroxidase activity increased by 1.58 U·mg-1. Compared with outdoor natural conditions, early diapause after 4℃ induction treatment, the activity of trehalase and pyruvate kinase were significantly decreased while the activity of peroxidase was significantly increased, however, the activity of sorbitol dehydrogenase remained stable under all induction temperatures. In different temperatures conditions, the activity of metabolic enzymes of soybean pod borer in early diapause(November), mid-diapause(February) and after the release of diapause(June) were significantly different. Among them, in the treatment group induced by 23℃, the trehalase, sorbitol dehydrogenase andpyruvate kinase enzyme activities peaked in those three diapause stages, February is the critical period when soybean pod borer changes from diapause to diapause termination.These results would provide basis for the occurrence and forecast of soybean pod borer in the future.

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Last Update: 2018-06-08