|Table of Contents|

Tolerance Screening of Wild Soybean to Glyphosate and the Changes of Chlorophyll Fluorescence Parameters after Glyphosate Application(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年03期
Page:
399-407
Research Field:
Publishing date:

Info

Title:
Tolerance Screening of Wild Soybean to Glyphosate and the Changes of Chlorophyll Fluorescence Parameters after Glyphosate Application
Author(s):
LIU Qi; XIANG Shi-gang; HU Yu-qi; QIANG Sheng; SONG Xiao-ling
(Weed Research Laboratory,College of Life Science of Nanjing Agricultural University,Nanjing 210095,China)
Keywords:
Wild soybean;  Glyphosate;  Chlorophyll fluorescence parameters;  Tolerant
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2018.03.0399
Abstract:
In order to study the difference of glyphosate tolerance of wild soybean from different populations and the effects of glyphosate on their fluorescence parameters, the glyphosate tolerance of different wild soybean populations which were sampled from 17 different sites were measured in greenhouse, and 4 populations with the highest, higher, lower, and lowest tolerance to glyphosate were selected to determine the changes of chlorophyll fluorescence parameters after spraying glyphosate. The results showed that, based on ED50 value, the population of Jintan (JT), Shucheng (SC), Tieling (TL) and Chenzhou (CZ) had the highest, higher, lower, and lowest tolerance to glyphosate and their tolerance index was 3.17, 2.17, 1.78 and 1, respectively. Two days after applying different doses of glyphosate, the maximal photochemical efficiency (Fv/Fm) and actual photochemical efficiency (Y(Ⅱ)) of PSⅡ, photochemical quenching (qP) and electron transport rate (ETR) of those 4 populations were generally decreased with the increasing glyphosate does. The non-photochemical quenching (NPQ) showed different trend of change in different populations, the NPQ of JT population increased while the NPQ of SC, TL and CZ population increased first and then decreased with the increasing glyphosate does. Consequently, after glyphosate application, the higher the tolerance, the smaller decline rate of Fv/Fm, Y (II), ETR and qP, and the more steady increasing of the NPQ.After the application of 1.2-2.4,0.6,0.6-1.8 and 0.6-1.2 kg·ha-1 active ingredients of glyphosate, the Fv/Fm,Y(Ⅱ), ETR and qP of JT population were significantly higher than that of other populations respectively.Therefore, after glyphosate application, comparing the difference of fluorescence parameters of different wild soybean populations could be used for rapid screening of glyphosate tolerance.

References:

［1］燕雪飞.中国野生大豆遗传多样性及其分化研究［D］.沈阳:沈阳农业大学,2014.(Yan X F.Genetic diversity and differentiation of the wild soybean (Glycine soja Sieb.et Zucc.)in China［D］.Shenyang:Shenyang Agricultural University,2014.)
［2］Fukuda Y.Cytogenetical studies on the wild and cultivated Manchurian soybeans.(Glycine L.)［J］.Japanese Journal of Botany,1933,6:489-506.
［3］李福山.大豆起源及其演化研究［J］.大豆科学,1994,13(1):61-66.(Li F S.Study on the origin and evolution of soybean［J］.Soybean Science,1994,13(1):61-66.)
［4］董英山,庄炳昌,赵丽梅,等.中国野生大豆遗传多样性中心［J］.作物学报,2000,26(5):521-527.(Dong Y S,Zhuang B C,Zhao L M,et al.The genetic diversity centers of annual wild soybean in China［J］.Acta Agronomica Sinica,2000, 26(5):521-527.)
［5］崔宁波,张正岩.转基因大豆研究及应用进展［J］.西北农业学报,2016,25(8):1111-1124.(Cui N B,Zhang Z Y.Advance of research and application of transgenic soybean［J］.Acta Agriculturae Boreali-occidentalis Sinica,2016,25(8):1111-1124.)
［6］王克晶,李福山.中国河北省和日本东北部天然野生大豆群体性状调查比较［J］.中国油料作物学报,2000,22(4): 17-22.(Wang K J,Li F S.Comparison of population characteristics about natural wild soybean in Hebei and Northeastern Japan［J］.Chinese Journal of Oil Crop Sciences,2000,22(4):17-22.)
［7］陈新,严继勇,高兵.野生大豆抗草甘膦基因漂移的初步研究［J］.中国油料作物学报,2004,26(2):89-91.(Chen X,Yan J Y,Gao B.Preliminary study on the drifting of glyphosate resistance gene in wild soybean［J］.Chinese Journal of Oil Crop Sciences, 2004,26(2):89-91.)
［8］刘杰,周波,杨春燕,等.抗草甘膦转EPSPS大豆的基因漂移研究［J］.大豆科学,2012,31(4):517-521.(Liu J,Zhou B,Yang C Y,et al.Gene flowing of genetically modified glyphosate-resistant soybean with EPSPS［J］.Soybean Science,2012,31(4):517-521.)
［9］周波,关荣霞,陶波,等.抗草甘麟转基因大豆基因逃逸检测及分子验证［C］.福建：全国作物生物技术与诱变技术学术研讨会论文摘要集,2005.(Zhou B,Guan R X,T B,et al.Detection of glyphosate-resistant transgenic soybean gene flowing and molecular identification［C］.Fujian:Summary of the 2005 National Symposium on Crop Biotechnology and Mutagenesis,2005.)
［10］崔云云,曹越平.抗草甘膦转基因大豆的获得［J］.上海交通大学学报(农业科学),2016,1(34),1-4.(Cao Y Y,Cao Y P.Obtaining of transgenic glyphosate-resistant soybean［J］.Journal of Shanghai Jiao Tong University (Agricultural Science),2016,1(34),1-4.)
［11］路超,袁建琴,马艳琴,等.转基因大豆的食用安全性研究进展［J］.农学学报,2015,12(5),82-85.(Lu C,Yuan J Q,Ma Y Q,et al.Research progress of food safety of genetically modified soybeans［J］.Journal of Agriculture,2015,12(5),82-85.)
［12］高越.抗草甘膦野生大豆种质挖掘及抗性机制研究［D］.哈尔滨:东北农业大学,2013.(Gao Y.Study on screening and resistant mechanism of germplasm resources to glyphosate-resistance in wild soybean［D］.Harbin:Northeast Agricultural University,2013.)
［13］原向阳,郭平毅,黄洁,等.缺磷胁迫下草甘膦对抗草甘膦大豆幼苗光合作用和叶绿素荧光参数的影响［J］.植物营养与肥料学报,2014,20(1):221-228.(Yuan X Y,Guo P Y,Huang J,et al.Effect of glyphosate on photosynthesis and chlorophyll fluorescence of leaves of glyphosate-resistant soybean ［Glycine max (L.) Merr.］seedlings under phosphorus deficiency stress［J］.2014,20(1):221-228.)
［14］许大全,张玉忠,张荣铣.植物光合作用的光抑制［J］.植物生理学通讯,1992,28(4):237-243.(Xu D Q,Zhang Y Z,Zhang R X.Photoinhibition of photosynthesis in plants［J］.Plant Physiology Communications,1992,28(4):237-243.)
［15］马玉心,崔大练.野生大豆与栽培大豆荧光特性比较研究［J］.大豆科学,2009,28(4):651-654.(Ma Y X,Cui D L.Comparison of chlorophyⅡ fluorescence parameters in Glycine soja and Giycine max［J］.Soybean Science,2009,28(4):651-654.)
［16］李强,王国祥,潘国权,等.水体浊度对菹草萌发及萌发苗光合荧光特性的影响［J］.生态学报,2006,26(11):3594-3601.(Li Q,Wang G X,Pan G Q,et al.Influence of water turbidity on germination of Potamogeton crispus and growth of germinated seedling［J］.Acta Ecologica Sinica,2006,26(11):3594-3601.)
［17］张守仁.叶绿素荧光动力学参数的意义及讨论［J］.植物学通报,1999,16(4):444-448.(Zhang S R.A discussion on chlorophyll fluorescence kinetics parameters and their significance［J］.Chinese Bulletin of Botany,1999,16(4):444-448.)
［18］高越.抗草甘膦野生大豆种质挖掘及抗性机制研究［D］.哈尔滨：东北农业大学,2013.(Gao Y.Study on screening and resistant mechanism of germplasm resources to glyphosate-resistance in wild soybean［D］.Harbin:Northeast Agricultural University,2013.)
［19］张静,唐继军,王岩,等.华北高寒区北沙参光合特性研究［J］.华北农学报,2011,26(2):218-222.(Zhang J,Tang J J,Wang Y,et al.The photosythetic characteristic of Glehnia littoralis in the northwest of Hebei［J］.Acta Agriculturae Boreali-Sinica,2011,26(2):218-222.)
［20］管铭,郭水良,裴立,等.基于光合和叶绿素荧光参数评判两个假稻种群对草甘膦的敏感性［J］. 上海师范大学学报 (自然科学), 2012, 41(2):171-178.(Guan M,Guo S L,Pei L,et al.Assessment on sensitiveness of leersia japonica to glyphosate based on photosynthetic and chlorophyll fluorescence parameters［J］.Journal of Shanghai Normal University(Natural Sciences),2012, 41(2):171-178.)
［21］Zhang T,Li F,Tian X,et al.Use of chlorophyll fluorescence and P700 absorbance to rapidly detect glyphosate resistance in goosegrass (Eleusine indica)［J］.Journal of Integrative Agriculture,2015,14(4):714-723.
［22］王正贵,周立云,郭文善,等.除草剂对小麦光合特性及叶绿素荧光参数的影响［J］.农业环境科学学报,2011,30(6):7-13.(Wang Z G,Zhou L Y,Guo W S,et al.Effects of herbicides on photosynthesis and chlorophyll fluorescence parameters in wheat leaves［J］.Journal of Agro-Environment Science,2011,30(6):7-13.)
［23］周朝彬,宋于洋,王炳举,等.干旱胁迫对胡杨光合和叶绿素荧光参数的影响［J］.西北林学院学报,2009,24(4):5-9.(Zhou C B,Song Y Y,Wang B J,et al.Effects of drought stress on photosynthesis and chlorophyll fluorescence parameters of populus euphratica［J］.Journal of Northwest Forestry University,2009,24(4):5-9.)
［24］Jin E S, Yokthongwattana K, Polle J E W, et al. Role of the reversible xanthophyll cycle in the photosystem II damage and repair cycle in Dunaliella salina［J］. Plant Physiology, 2003, 132(1): 352-364.
［25］何文铸,张彪,王培,等.利用叶绿素含量及荧光动力学参数评价青贮玉米耐旱关键指标研究［J］.干旱地区农业研究,2013,31(3):31-38.(He W Z,Zhang B,Wang P,et al.Evaluation of drought tolerance in silage maize based on chlorophyll content and fluorescence kinetics parameters［J］.Agricultural Research in the Arid Areas,2013,31(3):31-38.)
［26］童小芹,王淑智,夏咏,等.应用叶绿素荧光技术快速预警乌鲁木齐典型农作物干旱胁迫［J］.干旱区研究,2013,30(5):860-866.(Tong X Q,Wang S Z,Xia Y,et al.Early-warning of drought stress for typical crops in urumqi with chlorophyll fluorescence technique［J］.Arid Zone Research,2013,30(5):860-866.)
［27］张会慧,张秀丽,王娟,等.利用快相叶绿素荧光参数综合评价3种丁香的耐盐性［J］.南京林业大学学报(自然科学),2013,37(5):13-19.(Zhang H H,Zhang X L,Wang J,et al.A comprehensive evaluation of salt resistance in three clove varieties by the fast fluorescence transient parameters［J］.Journal of Nanjing Forestry University(Natural Science),2013,37(5):13-19.)
［28］黄有总,张国平.叶绿素荧光测定技术在麦类作物耐盐性鉴定中的应用［J］.麦类作物学报,2004,24(3):114-116.(Huang Y Z,Zhang G P.Application of measuring chlorophyll fluorescence in identification of salinity tolerance in triticeae crops［J］.Journal of Triticeae Crops,2004,24(3):114-116.)
［29］彭振,何守朴,孙君灵,等.陆地棉苗期耐盐性的高效鉴定方法［J］.作物学报,2014,40(3):476-486.(Peng Z,He S P,Sun J L,et al.An efficient approach to identify salt tolerance of upland cotton at seedling stage［J］.Acta Agronomica Sinica,2014,40(3):476-486.)
［30］姜建福,马寅峰,樊秀彩,等.196份葡萄属(Vitis L.)种质资源耐热性评价［J］.植物遗传资源学报,2017,18(1):70-79.(Jiang J F,Ma Y F,Fan X C,et al.Evaluation of 196 Vitis L.germplasm resources to heat tolerance［J］.Journal of Plant Geneti Resources,2017,18(1):70-79.)
［31］周桂英,王四清,许建新,等.8种大花蕙兰耐热性指标筛选及其评价［J］.安徽农业科学,2016,44(16):20-22.(Zhou G Y,Wang S Q,Xu J X,et al.Heat resistance indexes identification and comprehensive evaluation of 8 species of cymbidium hybridium［J］.Journal of Anhui Agricultural Sciences,2016,44(16):20-22.)

Memo

Memo:
-
Last Update: 2018-06-08