|Table of Contents|

Genetic Diversity of Small Seeded Soybean Varieties from Heilongjiang and Jilin Assessed by ISSR Markers(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年03期
Page:
347-352
Research Field:
Publishing date:

Info

Title:
Genetic Diversity of Small Seeded Soybean Varieties from Heilongjiang and Jilin Assessed by ISSR Markers
Author(s):
ZHENG Shu-bo1 YU Sheng-zhu2 ZHAO Hong-kun1 YUAN Cui-ping1 LIU Xiao-dong1 QI Guang-xun1 DONG Ying-shan1 WANG Yu-min1
(1.Jilin Academy of Agricultural Sciences, Changchun 130033, China; 2.College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China)
Keywords:
Small seeded soybean Genetic diversity ISSR marker
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2018.03.0347
Abstract:
24 different small seeded soybean varieties from Jilin and Heilongjiang provinces were analyzed by 10 ISSR markers which were selected from 100 UBC ISSR primers. The result showed that the polymorphic information content of 10 ISSR primers ranged from 0.211 2 to 0.310 3 with an average of 0.249 6. The ISSR marker indexes ranged from 1.093 8 to 4.954 9, with an average of 2.890 3. The genetic similarity coefficient between 24 small seeded soybean varieties varied from 0.666 7 to 0.926 2 with an average of 0.794 3. The genetic similarity coefficient range (0.690 7-0.926 2) of small seeded soybean varieties from Jilin province was greater than that (0.744 2-0.925 0) of small seeded soybean varieties from Heilongjiang. The average similarity coefficient of small seeded soybean varieties (0.798 2) from Jilin was slightly lower than that of small seeded soybean varieties (0.798 9) from Heilongjiang. This result indicated that the genetic variation of small seeded soybean varieties from Jilin province was more abundant. 4 clustering groups were suggested by clustering analysis. The small seeded soybean varieties in GroupⅠand GroupⅡ were all from Jilin province, Group Ⅲ could be divided into 2 subgroups. The result of principle coordinate analysis was consistent with that of cluster analysis. The results in this study could provide reference for genetic improvement of small seeded soybean.

References:

[1]史延茂,田智斌,张聪莎,等. 传统发酵大豆制品功能成分的研究进展[J].中国调味品,2012,37(12):13-20.(Shi Y M, Tian Z B, Zhang C S, et al. The development of traditional fermented soybean products functional components[J]. China Condiment, 2012,12(2):13-20.)
[2]肖伶俐,康玉凡,陶礼明.不同大豆品种芽用特性比较[J].大豆科学,2008,27(6): 955-959.(Xiao L L, Kang Y F, Tao L M, et al. Comparison analysis on characteristics of soybean growing sprouts for different soybean varieties[J]. Soybean Science, 2008, 27(6): 955-959.)
[3]王慧,马春梅,龚振平.大豆品种与豆芽营养品质及产量的关系研究[J].大豆科学,2014,6(3):374-378.(Wang H, Ma C M, Gong Z P. Study of relationship between soybean varieties and bean sprouts nutritional quality and yield [J]. Soybean Science, 2014,6(3):374-378.)
[4]花登峰, 赵团结, 张黎萍. 小粒专用大豆品种遗传改良研究进展[J]. 杂粮作物,2005(5):311-313.(Hua D F, Zhao T J, Zhang L P. Progress in genetic improvement of small seeded special soybean varieties[J]. Rain Fed Crops, 2005(5):311-313.)
[5]王昌陵,王文斌,董丽杰,等. 高产、优质小粒豆新品种辽小粒豆1号选育及配套栽培技术[J]. 辽宁农业科学,2014(2):76-77.(Wang C L, Wang W B, Dong L J, et al. Breeding of a new small seeded soybean variety of high yield and quality ‘Liao xiaolidou No. 1’ and its cultivation techniques[J]. Liaoning Agricultural Sciences, 2014(2):76-77.)[6]郑惠玉,杨光宇,韩春凤,等. 利用野生大豆(G.soja)种质育成小粒大豆新品种“吉林小粒1号”的选育报告[J]. 吉林农业科学,1991(3):9-11.(Zheng H Y, Yang G Y, Han C F, et al. The breeding report of small seeded soybean variety ‘Jilin XiaoLi 1’ by using wild soybean germplasm[J]. Journal of Jilin Agricultural Sciences, 1991(3):9-11.)
[7]郑淑波,赵洪锟,刘晓冬,等.不同小粒大豆品种豆芽特性比较[J]. 吉林农业科学,2013,38(5):81-85.(Zheng S B, Zhao H K, Liu X D, et al. Comparison on the sprout characteristics of different small seed soybean varieties[J]. Journal of Jilin Agricultural Sciences, 2013,38(5):81-85.)
[8]郑淑波.小粒大豆豆芽生产性能比较及遗传多样性分析[D]. 长春: 吉林农业大学, 2013: 10-33. (Zheng S B. Comparison of small soybean sprouts production performance and analysis of genetic diversity of the small soybean varieties [D]. Changchun: Jilin Agricultural University, 2013: 10-33.)
[9]Tel-Zur N, Abbo S, Myslabodski D, et al.Modified CTAB procedure for DNA isolation from epiphytic cacti of the genera hylocereus and selenicereus (Cactaceae)[J]. Plant Molecular Biology Reporter, 1999, 17(3): 249-254.
[10]Smith J S C, Chin E C L, Shu H, et al. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): Comparisons with data from RFLPs and pedigree[J]. Theoretical and Applied Genetics, 1997, 95(1-2):163-173.
[11]Rohlf F J. Numerical taxonomy and multivariate analysis system. NTSYS version 2.11a[M]. New York:Applied Biostatistics Inc,2002.
[12]Lam H M, Xu X, Liu X, et al. Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection [J]. Nature Genetics, 2010, 42(12):1053-1059.
[13]Zhou Z K, Jiang Y, Wang Z, et al. Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean [J]. Nature Biotechnology, 2015, 33(4): 408-414.
[14]吕祝章,王文哲,梁青,等.野生大豆育成品种与其亲本间的SSR聚类分析[J].湖北农业科学,2017,56(12): 2215-2218. (Lyu Z Z, Wang W Z, Liang Q, et al. SSR cluster analysis between cultivars and their parents in wild soybean[J].Hubei Agricultural Sciences, 2017,56(12):2215-2218.)
[15]杨光宇,王洋,马晓萍,等.野生大豆利用技术研究与应用[J]. 世界农业,2010(3):47-48.(Yang G Y, Wang Y, Ma X P, et al. Research and application of wild soybean utilization techniques[J]. World Agriculture, 2010 (3):47-48.)
[16]郭泰,王志新,吴秀红,等. 国外大豆资源利用与小粒大豆品种创新[J]. 中国农学通报,2009, 25(22): 306-310.(Guo T, Wang Z X, Wu X H, et al. The utilization of foreign soybean varieties resource and the innovation of small soybean varieties[J]. Chinese Agricultural Science Bulletin, 2009, 25(22):306-310.)

Memo

Memo:
-
Last Update: 2018-06-06