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Cloning and Prokaryotic Expression of GmPM13 Gene from Glycine max(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年02期
Page:
185-191
Research Field:
Publishing date:

Info

Title:
Cloning and Prokaryotic Expression of GmPM13 Gene from Glycine max
Author(s):
WANG Yu; LI Hong-li; WANG Jun-hao; LI Hai-yan; CUI Xi-yan
（School of Life Science，Jilin Agricultural University，Changchun 130118，China)
Keywords:
Glycine max;  GmPM13 gene;  Gene cloning;  Prokaryotic expression
PACS:
S565.1；Q785
DOI:
10.11861/j.issn.1000-9841.2018.02.0185
Abstract:
Through the cloning and prokaryotic expression of caleosin gene GmPM13 in soybean could provid the antibody for the detection of GmPM13 gene by genetic engineering in the future. GmPM13 gene of soybean caleosin was cloned by RT-PCR technology, and the prokaryotic expression vector was constructed which was named GmPM13. It was transformed into E.coli Rosetta with IPTG induction, the expression of GmPM13 protein was analyzed by SDS-PAGE. The full-length cDNA sequence of GmPM13 gene was 738 bp with a 720 bp ORF(open reading frame), encoding 239 amino acids. The molecular mass of the protein was 27.1 kDa, and the size of the induced product was consistent with the expected protein size. The induced protein content reached the highest level at 28℃, 1.5 mol·L-1 IPTG concentration, after induced 12 h, accounting for 39.25% in the total bacterial proteins.

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Last Update: 2018-04-02