|Table of Contents|

Isolation of Glyma08g11030 Promoter from F-box Gene of Glycine max and Construction of Its Plant Expression Vectors(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年02期
Page:
179-184
Research Field:
Publishing date:

Info

Title:
Isolation of Glyma08g11030 Promoter from F-box Gene of Glycine max and Construction of Its Plant Expression Vectors
Author(s):
ZHANG Cheng-qi; WANG Yi; XIA Cheng-lin; YU Yue-hua; NI Zhi-yong
（College of agronomy, Xinjiang Agricultural University, Urumqi 830052, China）
Keywords:
Soybean;  Promoter;  Clone;  Plant expression vector
PACS:
S565.1；Q78
DOI:
10.11861/j.issn.1000-9841.2018.02.0179
Abstract:
Based on the predicted sequence of Glyma08g11030 promoter region, primers were designed based on promoter sequence. We cloned 3 000 bp fragments from soybean Williams 82 genome DNA by PCR method.The promoter sequence was placed on the PlantCARE plant promoter region to predict online website queries. The predicted results showed that Glyma08g11030 promoter sequence contained a large number of cis-acting elements with various functions. The two basic cis-acting elements of TATA-box and CAAT-box were the most, and there were many cis-acting elements responding to it, such as drought, heat, light and hormones. According to the position of these cis-acting elements in the Glyma08g11030 promoter sequence, the primer sequences were truncated and the primers were designed respectively. Three truncated promoters were cloned successfully by PCR method and their length were 2 492, 1 185 and 342 bp. We constructed three GUS plant fusion expression vectors by replacing CaMV35S promoter of pCAMBIA3301 and obtained the promoter sequences with different cis-acting elements. These results would be helpful for the further research of functional characterization of cis-acting elements and regulation mechanism in Glyma08g11030 promoter.

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Last Update: 2018-04-02